Two-dimensional liquid chromatography separation of peptides using reversed-phase/weak cation-exchange mixed-mode column in first dimension
文献类型：期刊论文
| 作者 | Cai, Xiaoming; Guo, Zhimou; Xue, Xingya; Xu, Junyan; Zhang, Xiuli; Liang, Xinmiao |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2012-03-09 |
| 卷号 | 1228页码:242-249 |
| 关键词 | Two-dimensional liquid chromatography Mixed-mode chromatography Polar-copolymerized Proteomics Peptide separation LC/MS |
| 英文摘要 | selection of a first-dimensional separation method is very important in proteomic studies performed with two-dimensional liquid chromatography (2d-lc), since reversed-phase chromatography (rplc) is usually chosen as the second-dimensional separation method in most studies. in this paper, we assess the potential use of mixed-mode reversed-phase/weak cation-exchange (rp/wcx) chromatography in 2d-lc proteomic studies. first, a new rp/wcx mixed-mode stationary phase (named c18wcx) was synthesized based on the polar-copolymerized approach, and a c18wcx column with separation efficiency comparative to conventional c18 columns was developed. this new mixed-mode column primarily provides hydrophobic interactions under acidic condition, but can offer hydrophobic and cation-exchange interactions under neutral and weak basic conditions. an off-line 2d-rp/wcx-rplc system was established using the c18wcx column with a mobile phase of ph 6.5 in the first dimension and a 08 column with a mobile phase of ph 3.0 in the second dimension. the orthogonality of this two-dimensional system, evaluated through the separation of 123 tryptic peptides, was shown to be higher than that of the conventional 2d-rp-rplc approach. applying this 2d-lc method to rat brain samples, we identified 1031 proteins and 4397 unique peptides. in addition, this new two-dimensional method improved the identification of basic peptides. therefore, we propose that this novel 2d-rp/wcx-rplc system can be used as an alternative approach for the two-dimensional separation of peptides. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | protein identification technology ; tandem mass-spectrometry ; shotgun proteomics ; stationary-phase ; monolithic columns ; horizontal polymerization ; yeast proteome ; hilic-rp ; silica ; enrichment |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301406100024 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138026]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Xiaoming,Guo, Zhimou,Xue, Xingya,et al. Two-dimensional liquid chromatography separation of peptides using reversed-phase/weak cation-exchange mixed-mode column in first dimension[J]. journal of chromatography a,2012,1228:242-249. |
| APA | Cai, Xiaoming,Guo, Zhimou,Xue, Xingya,Xu, Junyan,Zhang, Xiuli,&Liang, Xinmiao.(2012).Two-dimensional liquid chromatography separation of peptides using reversed-phase/weak cation-exchange mixed-mode column in first dimension.journal of chromatography a,1228,242-249. |
| MLA | Cai, Xiaoming,et al."Two-dimensional liquid chromatography separation of peptides using reversed-phase/weak cation-exchange mixed-mode column in first dimension".journal of chromatography a 1228(2012):242-249. |
入库方式： OAI收割
来源：大连化学物理研究所
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