Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes
文献类型：期刊论文
| 作者 | Chang, Yuwei1; Zhao, Chunxia1; Zhu, Zhen2; Wu, Zeming1; Zhou, Jia1; Zhao, Yanni1; Lu, Xin1; Xu, Guowang1 |
| 刊名 | plant molecular biology
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 78期号:4-5页码:477-487 |
| 关键词 | Liquid chromatography Mass spectrometry Metabolic profiling Transgenic rice Unintended effects Metabolomics |
| 英文摘要 | as a primary characteristic of substantial equivalence, the evaluation of unintended effects of genetically modified plants has been evolving into an important field of research. in this study, a metabolic profiling method for rice seeds was developed using rapid resolution liquid chromatography/quadrupole time-of-flight mass spectrometry. the analytical properties of the method, including the linearity, reproducibility, intra-day precision and inter-day precision, were investigated and were found to be satisfactory. the method was then applied to investigate the differences between transgenic rice and its native counterparts, in addition to the differences found between native rice with different sowing dates or locations. global metabolic phenotype differences were visualized, and metabolites from different discriminated groups were discovered using multivariate data analysis. the results indicated that environmental factors played a greater role than gene modification for most metabolites, including tryptophan, 9,10,13-trihydroxyoctadec-11-enoic acid, and lysophosphatidylethanolamine 16:0. the concentrations of phytosphingosine, palmitic acid, 5-hydroxy-2-octadenoic acid and three other unidentified metabolites varied slightly due to gene modification. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; plant sciences |
| 研究领域[WOS] | biochemistry & molecular biology ; plant sciences |
| 关键词[WOS] | flight mass-spectrometry ; maize grain ; nontransgenic counterpart ; bacillus-thuringiensis ; safety evaluation ; oryza-sativa ; plants ; performance ; environment ; expression |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302252300012 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138029]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, Yuwei,Zhao, Chunxia,Zhu, Zhen,et al. Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes[J]. plant molecular biology,2012,78(4-5):477-487. |
| APA | Chang, Yuwei.,Zhao, Chunxia.,Zhu, Zhen.,Wu, Zeming.,Zhou, Jia.,...&Xu, Guowang.(2012).Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes.plant molecular biology,78(4-5),477-487. |
| MLA | Chang, Yuwei,et al."Metabolic profiling based on LC/MS to evaluate unintended effects of transgenic rice with cry1Ac and sck genes".plant molecular biology 78.4-5(2012):477-487. |
入库方式： OAI收割
来源：大连化学物理研究所
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