The Conformational Polymorphism of the Green Fluorescent Protein
文献类型：期刊论文
| 作者 | Tan, Haidong1; Li, Yueguang2; Chen, Ling3; Kudoh, Takayuki3; Kasai, Tomonari3; Seno, Masaharu3 |
| 刊名 | molecular biology
 |
| 出版日期 | 2012-02-01 |
| 卷号 | 46期号:1页码:142-148 |
| 关键词 | green fluorescent protein conformational polymorphism glutathione-S-transferase native polyacrylamide gel electrophoresis MALDI-TOF MS circular dichroism spectra |
| 英文摘要 | green fluorescent protein (gfpuv) has been widely used as a reporter fused to individual targeting sequences. however, its state in liquid and its effect on other proteins are still unclear. the conformational polymorphisms of glutathione-s-transferase-green fluorescent protein (gst-gfpuv), gfpuv and gst were analyzed by native polyacrylamide gel, indicating that gst was in many different states while gfpuv and gst-gfpuv were only in four and two slightly different states. four different circular dichroism spectra were obtained from the gfpuv polymorphisms. the single molecular behavior of gst-gfpuv and gfpuv was also characterized by maldi-tof ms. thus, we demonstrated that: (1) there might be four different structural polymorphisms for the native gfpuv; (2) gfpuv could reduce its partner's polymorphism as a fusion protein. although gfpuv had many merits as a reporter, its unreliability was found in the study. doi: 10.1134/s0026893311060045 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | living cells ; thermal-stability ; dynamics ; gfp ; spectroscopy ; expression ; molecules ; tracking ; reporter ; fusion |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302960100017 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138040]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.CAS, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Tianjin 4th Ctr Hosp, Dept Gen Surg, Tianjin 300140, Peoples R China 3.Okayama Univ, Grad Sch Nat Sci & Technol, Dept Med & Bioengn Sci, Okayama 7008530, Japan |
| 推荐引用方式 GB/T 7714 | Tan, Haidong,Li, Yueguang,Chen, Ling,et al. The Conformational Polymorphism of the Green Fluorescent Protein[J]. molecular biology,2012,46(1):142-148. |
| APA | Tan, Haidong,Li, Yueguang,Chen, Ling,Kudoh, Takayuki,Kasai, Tomonari,&Seno, Masaharu.(2012).The Conformational Polymorphism of the Green Fluorescent Protein.molecular biology,46(1),142-148. |
| MLA | Tan, Haidong,et al."The Conformational Polymorphism of the Green Fluorescent Protein".molecular biology 46.1(2012):142-148. |
入库方式： OAI收割
来源：大连化学物理研究所
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