Facile Preparation of Zwitterionic Organic-Silica Hybrid Monolithic Capillary Column with an Improved "One-Pot" Approach for Hydrophilic-Interaction Liquid Chromatography (HILIC)
文献类型：期刊论文
| 作者 | Lin, Hui1,2; Ou, Junjie1; Zhang, Zhenbin1,2; Dong, Jing1; Wu, Minghuo1,3; Zou, Hanfa1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2012-03-20 |
| 卷号 | 84期号:6页码:2721-2728 |
| 英文摘要 | a simple single-step thermal-treatment "one-pot" approach for the preparation of organic-silica hybrid capillary monolithic columns is described. in this improved method, the cross-linker vinyltrimethoxysilane (vtms) was replaced by 3-methacryloxypropyltrimethoxysilane (gamma-maps), which is more active in polymerization reactions, and only one thermal treatment step was required in the preparation of hybrid monoliths. two zwitterionic organic-silica monolithic columns were successfully synthesized by using [2-(methacryloyloxy)-ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (msa) and 2-methacryloyloxyethyl phosphorylcholine (mpc) as the organic monomers. the effects of the tetramethoxysilane (tmos)/gamma-maps molar ratio, content of monomer, composition of porogenic solvent, and reaction temperature on the morphologies of the hybrid monoliths were investigated. the msa-silica and mpc-silica hybrid monolithic columns exhibited good permeability and good mechanical stability. the monolithic columns were used for the separation of polar compounds by capillary hydrophilic-interaction chromatography (chilic). a typical hilic retention mechanism was observed at higher organic solvent contents (>50% acn). the msa monoliths were further investigated in the separation of various neutral, basic, and acidic analytes, as well as small peptides, by capillary liquid chromatography (clc), and high efficiency and satisfactory reproducibility were achieved. in addition, the analysis of a tryptic digest of bovine serum albumin (bsa) by clc tandem mass spectrometry (clc-ms/ms) with an msa monolith further demonstrated its potential in the separation of biological samples. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | polar stationary phases ; mass-spectrometry ; mixed-mode ; glycopeptide enrichment ; peptide separation ; proteome analysis ; n-glycopeptides ; nano-lc ; electrochromatography ; performance |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301634500016 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138054]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Nanchang Hangkong Univ, Coll Environm & Chem Engn, Nanchang 330063, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Hui,Ou, Junjie,Zhang, Zhenbin,et al. Facile Preparation of Zwitterionic Organic-Silica Hybrid Monolithic Capillary Column with an Improved "One-Pot" Approach for Hydrophilic-Interaction Liquid Chromatography (HILIC)[J]. analytical chemistry,2012,84(6):2721-2728. |
| APA | Lin, Hui,Ou, Junjie,Zhang, Zhenbin,Dong, Jing,Wu, Minghuo,&Zou, Hanfa.(2012).Facile Preparation of Zwitterionic Organic-Silica Hybrid Monolithic Capillary Column with an Improved "One-Pot" Approach for Hydrophilic-Interaction Liquid Chromatography (HILIC).analytical chemistry,84(6),2721-2728. |
| MLA | Lin, Hui,et al."Facile Preparation of Zwitterionic Organic-Silica Hybrid Monolithic Capillary Column with an Improved "One-Pot" Approach for Hydrophilic-Interaction Liquid Chromatography (HILIC)".analytical chemistry 84.6(2012):2721-2728. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。