Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor beta 1 (TGF-beta 1) in Epithelial Cell Lines
文献类型：期刊论文
| 作者 | Xu, Qingsong1,2; Isaji, Tomoya1; Lu, Yingying1; Gu, Wei1; Kondo, Madoka1; Fukuda, Tomohiko1; Du, Yuguang2; Gu, Jianguo1 |
| 刊名 | journal of biological chemistry
 |
| 出版日期 | 2012-05-11 |
| 卷号 | 287期号:20页码:16563-16574 |
| 英文摘要 | the epithelial-to-mesenchymal transition (emt) plays crucial roles in embryonic development, wound healing, tissue repair, and cancer progression. results of this study show how transforming growth factor beta 1 (tgf-beta 1) down-regulates expression of n-acetylglucosaminyltransferase iii (gnt-iii) during emt-like changes. treatment with tgf-beta 1 resulted in a decrease in e-cadherin expression and gnt-iii expression, as well as its product, the bisected n-glycans, which was confirmed by erythro-agglutinating phytohemagglutinin lectin blot and hplc analysis in human mcf-10a and mouse ge11 cells. in contrast with gnt-iii, the expression of n-acetylglucosaminyltransferase v was slightly enhanced by tgf-beta 1 treatment. changes in the n-glycan patterns on alpha 3 beta 1 integrin, one of the target proteins for gnt-iii, were also confirmed by lectin blot analysis. to understand the roles of gnt-iii expression in emt-like changes, the mcf-10a cell was stably transfected with gnt-iii. it is of particular interest that overexpression of gnt-iii influenced emt-like changes induced by tgf-beta 1, which was confirmed by cell morphological changes of phase contrast, immunochemical staining patterns of e-cadherin, and actin. in addition, gnt-iii modified e-cadherin, which served to prolong e-cadherin turnover on the cell surface examined by biotinylation and pulse-chase experiments. gnt-iii expression consistently inhibited beta-catenin translocation from cell-cell contact into the cytoplasm and nucleus. furthermore, the transwell assay showed that gnt-iii expression suppressed tgf-beta 1-induced cell motility. taken together, these observations are the first to clearly demonstrate that gnt-iii affects cell properties, which in turn influence emt-like changes, and to explain a molecular mechanism for the inhibitory effects of gnt-iii on cancer metastasis. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | e-cadherin ; glycosylation affects ; tumor progression ; breast-carcinoma ; propeller domain ; bisecting glcnac ; mouse melanoma ; expression ; adhesion ; catenin |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000304030900046 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138064]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Tohoku Pharmaceut Univ, Inst Mol Biomembrane & Glycobiol, Div Regulatory Glycobiol, Aoba Ku, Sendai, Miyagi 9818558, Japan 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Qingsong,Isaji, Tomoya,Lu, Yingying,et al. Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor beta 1 (TGF-beta 1) in Epithelial Cell Lines[J]. journal of biological chemistry,2012,287(20):16563-16574. |
| APA | Xu, Qingsong.,Isaji, Tomoya.,Lu, Yingying.,Gu, Wei.,Kondo, Madoka.,...&Gu, Jianguo.(2012).Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor beta 1 (TGF-beta 1) in Epithelial Cell Lines.journal of biological chemistry,287(20),16563-16574. |
| MLA | Xu, Qingsong,et al."Roles of N-Acetylglucosaminyltransferase III in Epithelial-to-Mesenchymal Transition Induced by Transforming Growth Factor beta 1 (TGF-beta 1) in Epithelial Cell Lines".journal of biological chemistry 287.20(2012):16563-16574. |
入库方式： OAI收割
来源：大连化学物理研究所
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