Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides
文献类型：期刊论文
| 作者 | Li, Qin1,2; Lynen, Frederic1; Wang, Jian3; Li, Hanlin3; Xu, Guowang2; Sandra, Pat1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2012-09-14 |
| 卷号 | 1255页码:237-243 |
| 关键词 | Comprehensive two-dimensional liquid chromatography Hydrophilic interaction liquid chromatography Ion-pair reversed-phase liquid chromatography LC x LC interface Oligonucleotides |
| 英文摘要 | a comprehensive two-dimensional hplc approach with a high degree of orthogonality was developed for analysis of di- to deca-oligonucleotides (ons). hydrophilic interaction liquid chromatography (hilic) was used in the first dimension, and ion-pair reversed-phase liquid chromatography (ip-rplc) was employed in the second dimension. the two dimensions were connected via a ten-port valve interface equipped with octadecyl silica (ods) traps to immobilize and focus the ons eluting from the first dimension prior to ip-rplc separation. an aqueous make-up flow was used for effective trapping. the comprehensive two-dimensional hplc system was optimized with a mixture consisting of 27 oligonucleotide standards. an overall chromatographic peak capacity of 500 was obtained. the use of the volatile buffer triethylamine acetate in the second dimension allowed straightforward coupling to electrospray ionization mass spectrometry (esi-ms) and detection of each on in the negative ionization mode. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | anion-exchange chromatography ; capillary gel-electrophoresis ; mass-spectrometry ; phosphorothioate oligonucleotides ; biological-fluids ; interfering rna ; nucleic-acids ; performance ; separation ; purification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308524600024 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138086]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Ghent, Pfizer Analyt Res Centre, B-9000 Ghent, Belgium 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Pfizer Inc, Pharmaceut Sci, Groton, CT 06340 USA |
| 推荐引用方式 GB/T 7714 | Li, Qin,Lynen, Frederic,Wang, Jian,et al. Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides[J]. journal of chromatography a,2012,1255:237-243. |
| APA | Li, Qin,Lynen, Frederic,Wang, Jian,Li, Hanlin,Xu, Guowang,&Sandra, Pat.(2012).Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides.journal of chromatography a,1255,237-243. |
| MLA | Li, Qin,et al."Comprehensive hydrophilic interaction and ion-pair reversed-phase liquid chromatography for analysis of di- to deca-oligonucleotides".journal of chromatography a 1255(2012):237-243. |
入库方式： OAI收割
来源：大连化学物理研究所
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