Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography
文献类型：期刊论文
| 作者 | Wang, HL; Zou, HF; Zhang, YK |
| 刊名 | biomedical chromatography
 |
| 出版日期 | 1998 |
| 卷号 | 12期号:1页码:4-7 |
| 关键词 | microdialysis fenoprofen human serum albumin multi-site binding high performance liquid chromatography competitive interaction |
| 英文摘要 | a simple and fast method for the determination of the multi-site binding of fenoprofen (fp) to human serum albumin (hsa) has been developed by utilizing microdialysis sampling techniques combined with high performance liquid chromatography (hplc), the drug and protein were mixed in different molar ratios in 0.067 mol potassium phosphate buffer, ph 7.4, and incubated at 37 degrees c in a water-bath. then the microdialysis probe was put in the fp-hsa solution and sampled at the perfusion rate of 1 mu l/min. the concentrations of fp in microdialysates were determined by the reversed-phase high performance liquid chromatography, relative recovery (r) was also determined in vitro on similar condition, r is about 56.03+/-1.11% (n=3). fenoprofen was found to bind to two classes of sites, the association constant (k-1) and the number of the binding sites on primary binding sites of a hsa molecule (n(1)) for fenoprofen are 3.4 x 10(5)/m and 2.5, respectively, and those for secondary binding are 1.0 x 10(4)/m and 10.0, respectively, the competitive interaction of ibuprofen (if) and palmitic acid with fenoprofen to hsa were also studied, both compounds significantly decrease the binding degree of fenoprofen to hsa. (c) 1998 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | plasma-protein binding ; frontal analysis ; capillary electrophoresis ; stationary phase ; silica column ; drugs ; acid ; carbamazepine |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000071630400002 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138166]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, HL,Zou, HF,Zhang, YK. Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography[J]. biomedical chromatography,1998,12(1):4-7. |
| APA | Wang, HL,Zou, HF,&Zhang, YK.(1998).Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography.biomedical chromatography,12(1),4-7. |
| MLA | Wang, HL,et al."Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography".biomedical chromatography 12.1(1998):4-7. |
入库方式： OAI收割
来源：大连化学物理研究所
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