Effects of experimental parameters on the signal intensity of capillary electrophoresis electrospray ionization mass spectrometry in protein analysis
文献类型：期刊论文
| 作者 | Liang, Z; Yang, Q; Zhang, WB; Zhang, LH; Zhang, YK |
| 刊名 | chromatographia
 |
| 出版日期 | 2003-05-01 |
| 卷号 | 57期号:9-10页码:617-621 |
| 关键词 | capillary electrophoresis electrospray ionization-mass spectrometry interface conditions proteins |
| 英文摘要 | in recent years, the hyphenation of capillary electrophoresis (ce) and electrospray ionization mass spectrometry (esi-ms) has been widely used for the analysis of biological molecules with high efficiency and high accuracy however, the signal intensity of ce-esi-ms is restricted by various parameters. this paper reports the effects on the ce-esi-ms signal of the ph and the electrolyte concentration, the formic acid concentration of the sheath liquid, and the sheath liquid composition, using several proteins as samples. the study was performed systematically by experimental and theoretical analyses. the maximum signal intensity of three proteins (cytochrome c, insulin, bovine serum albumin) was attained with a ph 4.40 buffer containing 75 mm formic acid and 75 mm ammonium acetate. investigation of the influence of the formic acid concentration in the sheath liquid (over the range of 0%-1%) on the esi-ms signal of brovine serum albumin showed that the feasible amount of the formic acid in sheath liquid could improve the signal intensity of the sample ions. however, considerable bond broadening was observed that should be attributed mainly to column overloading, band spreading at the interface, and scanning data acquisition. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | interface ; peptides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183320400008 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138255]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Z,Yang, Q,Zhang, WB,et al. Effects of experimental parameters on the signal intensity of capillary electrophoresis electrospray ionization mass spectrometry in protein analysis[J]. chromatographia,2003,57(9-10):617-621. |
| APA | Liang, Z,Yang, Q,Zhang, WB,Zhang, LH,&Zhang, YK.(2003).Effects of experimental parameters on the signal intensity of capillary electrophoresis electrospray ionization mass spectrometry in protein analysis.chromatographia,57(9-10),617-621. |
| MLA | Liang, Z,et al."Effects of experimental parameters on the signal intensity of capillary electrophoresis electrospray ionization mass spectrometry in protein analysis".chromatographia 57.9-10(2003):617-621. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。