Simultaneous detection of several single nucleotide polymorphisms
文献类型：期刊论文
| 作者 | Zhao, CX; Shi, XZ; Zhang, Y; Ma, JM; Mou, HM; Lu, S; Xu, GW |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2003-08-01 |
| 卷号 | 31期号:8页码:906-910 |
| 关键词 | single nucleotide polymorphism single nucleotide polymorphism analysis |
| 英文摘要 | accurate and fast genotyping of single nucleotide polymorphisms (snps) is of significant scientific importance in human genome project. here a new automated fluorescent method that can rapidly and accurately genotype multiplex known snps was developed, which was achieved by substantially improving a commercially available protocol. by this method, mismatch repair gene hmlh1 and cancer repression gene p53 were investigated, eight known snp spots (including five hot spots) of 20 coloeretal cancer patients' hmlh1 and p53 gene were detected, and a heterozygosity mutation (gtt-->gat) at codon 384 of hmlh1 was found. sequencing result of hmlh1 proves the reliability of this new method. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | capillary-electrophoresis ; human-genome ; mutation ; cancer ; hmlh1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185102000003 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138267]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 2.Dalian Med Univ, Attached Teaching Hosp 2, Cell Lab, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, CX,Shi, XZ,Zhang, Y,et al. Simultaneous detection of several single nucleotide polymorphisms[J]. chinese journal of analytical chemistry,2003,31(8):906-910. |
| APA | Zhao, CX.,Shi, XZ.,Zhang, Y.,Ma, JM.,Mou, HM.,...&Xu, GW.(2003).Simultaneous detection of several single nucleotide polymorphisms.chinese journal of analytical chemistry,31(8),906-910. |
| MLA | Zhao, CX,et al."Simultaneous detection of several single nucleotide polymorphisms".chinese journal of analytical chemistry 31.8(2003):906-910. |
入库方式： OAI收割
来源：大连化学物理研究所
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