Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography
文献类型：期刊论文
| 作者 | Jin, WH; Fu, HJ; Huang, XD; Xiao, H; Zou, HF |
| 刊名 | electrophoresis
 |
| 出版日期 | 2003-09-01 |
| 卷号 | 24期号:18页码:3172-3180 |
| 关键词 | basic pharmaceuticals capillary electrochromatography hormones peptides stationary phase styrene-based monolith |
| 英文摘要 | preparation of a poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic stationary phase for the use in capillary electrochromatography (cec) has been improved by optimizing the polymerization conditions. it is observed that the reaction time strongly affects column efficiency, while the proportion of isooctane in porogen influences peak symmetry of some solutes seriously. the lifetime of the monolithic columns prepared mainly depends on the ph of buffers used. reproducibility of electroosmotic flow (eof) from batch to batch columns are lower than 2.8% relative standard deviation. unlike other types of capillary electrochromatographic monoliths, a ph-dependent eof was observed on this type of column. separation of various types of compounds including aromatic hydrocarbons, hormones, anilines, basic pharmaceuticals, and peptides was achieved. the facile preparation and wide application of this monolithic column may make styrene-based polymer a potential stationary phase in cec. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | rigid polymer monoliths ; reversed-phase electrochromatography ; stationary phases ; liquid-chromatography ; separation media ; packed capillaries ; porous properties ; surface-chemistry ; peptides ; flow |
| 收录类别 | ISTP ; SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185897100011 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138320]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, WH,Fu, HJ,Huang, XD,et al. Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography[J]. electrophoresis,2003,24(18):3172-3180. |
| APA | Jin, WH,Fu, HJ,Huang, XD,Xiao, H,&Zou, HF.(2003).Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography.electrophoresis,24(18),3172-3180. |
| MLA | Jin, WH,et al."Optimized preparation of poly(styrene-co-divinylbenzene-co-methacrylic acid) monolithic capillary column for capillary electrochromatography".electrophoresis 24.18(2003):3172-3180. |
入库方式： OAI收割
来源：大连化学物理研究所
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