Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine
文献类型：期刊论文
| 作者 | Mei, SR; Yao, CH; Cai, LS; Xing, J; Xu, GW; Wu, CY |
| 刊名 | electrophoresis
 |
| 出版日期 | 2003-05-01 |
| 卷号 | 24期号:9页码:1411-1415 |
| 关键词 | cancer capillary electrophoresis end-column amperometric detection 8-hydroxy-2 '-deoxyguanosine urine |
| 英文摘要 | urinary 8-hydroxy-2'-deoxyguanosine (80hdg) is an excellent marker of oxidative dna damage. until now, urinary 80hdg has been measured by high-performance liquid chromatography with electrochemical detection. a simple and sensitive method for the analysis of urinary 80hdg by capillary electrophoresis with end-column amperometric detection has been developed in our laboratory. a single-step solid-phase extraction procedure was optimized and used for extracting 80hdg from human urine. to improve the sensitivity of this method, a new focusing technique based on a dynamic ph junction was used. the limit of detection was 20 nm (signal-to-noise ratio s/n = 3), the linear range was 50 nm-10 mum, and the correlation coefficient was better than 0.999. the relative standard deviation (rsd) was found to be 0.57% for migration time, and 4.79% for peak current. to show the usefulness of the method, the urinary concentration of 80hdg in nine healthy persons and ten cancer patients was determined. the urinary concentration of 80hdg in cancer patients was significantly higher than that in healthy persons. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | oxidative dna-damage ; dynamic ph junction ; electrochemical detection ; gas-chromatography ; 8-hydroxydeoxyguanosine ; mutations ; biomarker ; assay ; base |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000182965000011 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138353]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, R&A Ctr, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mei, SR,Yao, CH,Cai, LS,et al. Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine[J]. electrophoresis,2003,24(9):1411-1415. |
| APA | Mei, SR,Yao, CH,Cai, LS,Xing, J,Xu, GW,&Wu, CY.(2003).Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine.electrophoresis,24(9),1411-1415. |
| MLA | Mei, SR,et al."Capillary electrophoresis with end-column amperometric detection of urinary 8-hydroxy-2 '-deoxyguanosine".electrophoresis 24.9(2003):1411-1415. |
入库方式： OAI收割
来源：大连化学物理研究所
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