Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions
文献类型：期刊论文
| 作者 | Zheng, XF; Wu, L; Gao, DB; Chi, NY; Lin, QS; Hu, JH |
| 刊名 | spectrochimica acta part a-molecular and biomolecular spectroscopy
 |
| 出版日期 | 2003-06-01 |
| 卷号 | 59期号:8页码:1751-1755 |
| 关键词 | EPR spectroscopy metalloenzyme interaction Cu(II) |
| 英文摘要 | in the past. the method of reconstitution was used to investigate the interaction between metalloenzymes (containing zn(11)) and metal ions. in this paper, electron paramagnetic resonance (epr) has been employed to firstly study the direct interactions between bacillus subtilis neutral proteinase (bsnp), nuclease pi and cu(ii) ions added in aqueous solution, respectively. these results show that a dynamic equilibrium exists between the zn(ii) in the active site of native enzymes and the added cu(ii), the added cu(ii) partly replaces the zn(ii), forming cu(ii)-enzyme derivatives. as a result, the activity of the native enzymes is influenced. the influences of ph value on this kind of interaction have also been investigated, and the results demonstrate that the change of ph value has little influence on the system of nuclease p1, but has remarkable influence on bsnp. we firstly obtained the epr spectra for cu(ii)-enzyme derivatives. in addition, the derivative of cu(ii)-bsnp exists in the solution with two different conformations (i type g(parallel to) = 2.34, a(parallel to) (mt) = 13.4; 11 type g(parallel to) = 2.25, a(parallel to) (mt) = 16.1), and this two conformations exchanged each other depending on ph. (c) 2002 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; technology |
| 类目[WOS] | spectroscopy |
| 研究领域[WOS] | spectroscopy |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183006700011 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138378]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, Coll Bioengn, Dalian 116627, Peoples R China 2.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China 3.Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, XF,Wu, L,Gao, DB,et al. Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions[J]. spectrochimica acta part a-molecular and biomolecular spectroscopy,2003,59(8):1751-1755. |
| APA | Zheng, XF,Wu, L,Gao, DB,Chi, NY,Lin, QS,&Hu, JH.(2003).Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions.spectrochimica acta part a-molecular and biomolecular spectroscopy,59(8),1751-1755. |
| MLA | Zheng, XF,et al."Applications of electron paramagnetic resonance spectroscopy to study interactions of metalloenzymes with Cu(II) ions".spectrochimica acta part a-molecular and biomolecular spectroscopy 59.8(2003):1751-1755. |
入库方式： OAI收割
来源：大连化学物理研究所
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