Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase
文献类型：期刊论文
| 作者 | Ping, GC; Zhang, WB; Zhang, LH; Schmitt-Kopplin, P; Zhang, YK; Kettrup, A |
| 刊名 | chromatographia
 |
| 出版日期 | 2003-05-01 |
| 卷号 | 57期号:9-10页码:629-633 |
| 关键词 | capillary electrochromatography monolithic column nucleosides |
| 英文摘要 | separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic column is described. because the nucleosides are relatively hydrophilic compounds, chromatographic partition is very weak on non-polar stationary phases. a small quantity of organic modifier is usually required to increase their retention factors but, because the wettability of the stationary phase by the mobile phase is rather poor, bubble formation can be a significant problem. this situation was improved by use of a polymeric monolithic column with higher level charged monomer in the polymerization mixture, because of the presence of charged moieties on the surface of the monolithic stationary phase. the very high stability under conditions of very low organic modifier concentration used in the experiment showed that the wettability of the stationary phase by the mobile phase was satisfactory. the effect of ph, organic modifier content, and applied voltage on the separation was investigated. baseline separation of six nucleosides was achieved within 4.5 min on this type of column. because of the presence of charged moieties and butyl groups on the surface of the monolithic column, the mechanism of separation might involve both ion-exchange and hydrophobic interactions. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; rigid polymer monoliths ; columns ; media |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183320400010 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138390]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China 2.GSF Natl Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany |
| 推荐引用方式 GB/T 7714 | Ping, GC,Zhang, WB,Zhang, LH,et al. Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase[J]. chromatographia,2003,57(9-10):629-633. |
| APA | Ping, GC,Zhang, WB,Zhang, LH,Schmitt-Kopplin, P,Zhang, YK,&Kettrup, A.(2003).Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase.chromatographia,57(9-10),629-633. |
| MLA | Ping, GC,et al."Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase".chromatographia 57.9-10(2003):629-633. |
入库方式： OAI收割
来源：大连化学物理研究所
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