Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers
文献类型：期刊论文
| 作者 | Yang, L; Jia, LY; Zou, HF; Zhou, DM; Zhang, YK |
| 刊名 | science in china series b-chemistry
 |
| 出版日期 | 1998-12-01 |
| 卷号 | 41期号:6页码:596-605 |
| 关键词 | membrane affinity chromatography matrix separation biopolymers |
| 英文摘要 | a new iminodiacetic acid (ida) type chelating membrane based on composite matrix formed by covalently coupling the polyglycidyl methacrylate to cotton cellulose fiber was developed. the study focused on the optimal conditions for the preparation of the membrane and the reaction of the chelating agent (iminodiacetic acid, ida) with the matrix. the physical properties of the chelating membrane were tested by electron micrography and bet surface area instrument. the relationship between flow-rate and backpressure was almost linear. adsorption isotherm of protein on cu2+ - ida membrane was determined by batch experiments. the dissociation constants and the maximum adsorption capacity were obtained. the results of affinity purification of human serum albumin and bovine liver catalase on membrane are comparable with those on gel chromatography presented in literature. the separations performed on the membrane chromatography, however, were 3-5 times faster than those on gel chromatography. the immobilized metal composite membrane was also successfully used in hplc for rapid analysis of protein. thus, the composite membrane reported here, with low backpressure, low cost and long service-life, is quite suitable for rapid and large scale affinity purification and hplc analysis of biopolymers. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | chromatography ; purification ; proteins |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000077599300005 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138470]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, L,Jia, LY,Zou, HF,et al. Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers[J]. science in china series b-chemistry,1998,41(6):596-605. |
| APA | Yang, L,Jia, LY,Zou, HF,Zhou, DM,&Zhang, YK.(1998).Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers.science in china series b-chemistry,41(6),596-605. |
| MLA | Yang, L,et al."Immobilized metal affinity composite membrane based on cellulose for separation of biopolymers".science in china series b-chemistry 41.6(1998):596-605. |
入库方式： OAI收割
来源：大连化学物理研究所
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