Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells
文献类型：期刊论文
| 作者 | Zhang, W; Seki, M; Furusaki, S; Middelberg, APJ |
| 刊名 | journal of fermentation and bioengineering
 |
| 出版日期 | 1998 |
| 卷号 | 86期号:1页码:72-78 |
| 关键词 | strawberry suspension culture anthocyanin kinetics nutrient uptake mathematical model |
| 英文摘要 | strawberry (fragaria ananassa cv. shikinari) cell suspension cultures carried out in shake flasks for 18 d were closely examined for cell growth, anthocyanin synthesis and the development of pigmented cells in relation to the uptake of carbohydrate, extracellular po4, no3, nh4, and calcium. cell viability, extracellular anthocyanin content, ph and electrical conductivity of the broth were also monitored. the specific growth rate of strawberry cells at exponential phase was 0.27 and 0.28 d(-1) based on fresh and dry weight, respectively. anthocyanin synthesis was observed to increase continuously to a maximum value of 0.86 mg/g fresh cell weight (fcw) at day 6, and was partially growth-associated. anthocyanin synthesis was linearly related to the increase in pigmented cell ratio, which increased with time and reached a maximum value of ca. 70% at day 6 due to reduction in cell viability and depletion of substrate. total carbohydrate uptake was closely associated with increase in cell growth, and glucose was utilized in preference to fructose. nitrate and ammonia were consumed until 9 d of culture, but phosphate was completely absorbed within 4 d. calcium was assimilated throughout the growth cycle. after 9 d, cell lysis was observed which resulted in the leakage of intracellular substances and a concomitant ph rise. anthocyanin was never detected in the broth although the broth became darkly pigmented during the lysis period. this suggests that anthocyanin was synthesized only by viable pigmented cells, and degraded rapidly upon cell death and lysis. based on the results of kinetic analysis, a model was developed by incorporating governing equations for the ratio of pigmented cells into a bailey and nicholson's model. this was verified by comparison with the experimental data. the results suggest bat the model satisfactorily describes the strawberry cell culture process, and may thus be used for process optimization. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology ; food science & technology |
| 研究领域[WOS] | biotechnology & applied microbiology ; food science & technology |
| 关键词[WOS] | viability assay ; carrot cells ; plant-cells ; vitis sp ; identification ; kinetics ; chloride ; tissue ; model ; sugar |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000075734500012 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138514]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biochem Engn, Dalian 116012, Peoples R China 3.Univ Adelaide, Dept Chem Engn, Adelaide, SA 5005, Australia |
| 推荐引用方式 GB/T 7714 | Zhang, W,Seki, M,Furusaki, S,et al. Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells[J]. journal of fermentation and bioengineering,1998,86(1):72-78. |
| APA | Zhang, W,Seki, M,Furusaki, S,&Middelberg, APJ.(1998).Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells.journal of fermentation and bioengineering,86(1),72-78. |
| MLA | Zhang, W,et al."Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells".journal of fermentation and bioengineering 86.1(1998):72-78. |
入库方式： OAI收割
来源：大连化学物理研究所
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