Membrane affinity chromatography for analysis and purification of biopolymers
文献类型：期刊论文
| 作者 | Zhou, DM; Zou, HF; Ni, JY; Wang, HL; Yang, L; Zhang, YK |
| 刊名 | chromatographia
 |
| 出版日期 | 1999-07-01 |
| 卷号 | 50期号:1-2页码:27-34 |
| 关键词 | column liquid chromatography membrane stationary phase immunoaffinity analysis affinity efficiency adsorption capacity |
| 英文摘要 | affinity columns suitable for hplc were prepared by immobilization of various ligands of protein a, human igg, human igm and pectinase on gma modified cellulose membrane. the adsorption capacity, affinity efficiency and activity recovery of various iggs on these affinity columns were measured. it was observed that the length of the coupling arm plays a very important role in affinity efficiency, and the effect of eluent flow-rate on adsorption capacity was very small. the protein a column was exploited for the process monitoring of dog igg in clinical experiments on immuno-adsorption therapy. a pectinase column was used for the determination of polygalacturonase inhibiting proteins first purified on a hydroxyapatite column. it took only about 2.5 min for analysis at a flow-rate of 1.0 ml min(-1). the high speed analysis of biopolymers could be performed at a flow rate of 6.0 ml min(-1) within 15 s. membrane affinity chromatography gives good reproducibility, high efficiency, low column-pressure and is rapid. it can also be used for micro-scale purification of biopolymers. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | biomolecules ; performance ; proteins ; removal |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000081720200006 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138576]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, DM,Zou, HF,Ni, JY,et al. Membrane affinity chromatography for analysis and purification of biopolymers[J]. chromatographia,1999,50(1-2):27-34. |
| APA | Zhou, DM,Zou, HF,Ni, JY,Wang, HL,Yang, L,&Zhang, YK.(1999).Membrane affinity chromatography for analysis and purification of biopolymers.chromatographia,50(1-2),27-34. |
| MLA | Zhou, DM,et al."Membrane affinity chromatography for analysis and purification of biopolymers".chromatographia 50.1-2(1999):27-34. |
入库方式： OAI收割
来源：大连化学物理研究所
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