Investigation of molecular association by capillary zone electrophoresis frontal analysis
文献类型：期刊论文
| 作者 | Ding, YS; Lin, BC |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 1999 |
| 卷号 | 27期号:1页码:20-23 |
| 关键词 | capillary electrophoresis frontal analysis propranolol human serum albumin association parameters |
| 英文摘要 | a capillary zone electrophoresis/frontal analysis method was discussed and applied to the study of association parameters of a basic bug, propranolol (pro), to human serum albumin (hsa) under drug-hsa interaction equilibra. after the direct hydrodynamic injection (110 kpa. s) of a drug-hsa mixture into a coated capillary (effective length, 32 cm; 50 mu m i.d.), the basic bug is eluted as a zonal peak with a plateau region under condition of phosphate buffer ( ph 7.4, ionic strength = 0.17) at 10 kv running voltage. the unbound concentration of bug can be determined from the plateau peak height with good linear response, r = 0.9991 (n = 8). eight pro-hsa solutions (containing 100 similar to 450 mu mol/l of pro and 400 similar to 50 mu mol/l, of hsa, respectively) were investigated. the obtained data were processed with scatchard linear regression analysis and the final estimated pro-hsa association parameters include binding sites n = 0.58 (+/- 0.03) and binding constant k = 1500(+/- 110). |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | binding constants ; ligands ; drug |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000080031800005 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138598]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, YS,Lin, BC. Investigation of molecular association by capillary zone electrophoresis frontal analysis[J]. chinese journal of analytical chemistry,1999,27(1):20-23. |
| APA | Ding, YS,&Lin, BC.(1999).Investigation of molecular association by capillary zone electrophoresis frontal analysis.chinese journal of analytical chemistry,27(1),20-23. |
| MLA | Ding, YS,et al."Investigation of molecular association by capillary zone electrophoresis frontal analysis".chinese journal of analytical chemistry 27.1(1999):20-23. |
入库方式： OAI收割
来源：大连化学物理研究所
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