Polymerase chain reaction product analysis by capillary electrophoresis in a denatured non-gel sieving matrix
文献类型：期刊论文
| 作者 | Han, FT; Xu, Q; Lin, BC; Shen, Y; Wu, GY |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 1999-10-01 |
| 卷号 | 27期号:10页码:1179-1182 |
| 关键词 | capillary electrophoresis polymerase chain reaction non-gel sieving |
| 英文摘要 | the ordinary method of polymerase chain reaction (pcr) product identification is to let the pcr product comigrate with some standard deoxyribonucleic acid (dna) fragments, the length of the pcr product can be estimated by the migration time. undenatured buffer or normal buffer systems were often employed to separate dna fragments. because the migration behavior of dna fragments in the capillary was determined by fragment length as well as conformation, denaturant such as urea should be used in the buffer to eliminate the influence of conformation. the author found that when the pcr products of sry (sex-determining region of the y chromosome) and inner contrast 108/b comigrated with dna standards pbr322/hae iii, peak reversion can be noticed. this kind of peak reversion is the result of conformation influence. when estimating the length of the pcr product by calibrating method at this time, false result may be obtained. in a denatured buffer contained urea as denaturant, the pcr products appear at the right place in the electropherogram, which indicates that dna migrates only according to its fragment length. a more precise result can be obtained when estimating the length of the fragments in a denatured non-gel sieving system. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000083377800015 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138614]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Med Sci, Inst Basic Med, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, FT,Xu, Q,Lin, BC,et al. Polymerase chain reaction product analysis by capillary electrophoresis in a denatured non-gel sieving matrix[J]. chinese journal of analytical chemistry,1999,27(10):1179-1182. |
| APA | Han, FT,Xu, Q,Lin, BC,Shen, Y,&Wu, GY.(1999).Polymerase chain reaction product analysis by capillary electrophoresis in a denatured non-gel sieving matrix.chinese journal of analytical chemistry,27(10),1179-1182. |
| MLA | Han, FT,et al."Polymerase chain reaction product analysis by capillary electrophoresis in a denatured non-gel sieving matrix".chinese journal of analytical chemistry 27.10(1999):1179-1182. |
入库方式： OAI收割
来源：大连化学物理研究所
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