Covalent immobilization of trypsin on glycidyl methacrylate-modified cellulose membrane as enzyme reactor
文献类型：期刊论文
| 作者 | Jiang, HH; Zou, HF; Wang, HL; Ni, JY; Zhang, Q |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2000-05-01 |
| 卷号 | 21期号:5页码:702-706 |
| 关键词 | glycidyl methacrylate(GMA) -modified cellulose membrane immobilized trypsin enzyme biological reactor degradation of protein MALDI-TOF-MS cytochrome C |
| 英文摘要 | the membrane of the activated glycidyl methacrylate (gma)-modified cellulose was prepared and packed into a column piece by piece to make a microreactor by immobilization of trypsin. the microreactor based on the membrane medium showed the advantages of being cheaper, mechanically strong and chemically stable. the activity of immobilized trypsin towards n-benzoyl-l-arginine ethyl ester(baee) was 17 800 u/g dry membrane, and was 52% of that for free enzyme. besides, the effects of ph value of buffer, temperature, ionic strength, organic modifier, and protein denaturants on the activity of immobilized trypsin were investigated in comparison to the free trypsin, and thermal stability also was found especially to be improved after immobilization. the activity of the immobilized trypsin showed no decay after continuously pumping baee through immobilized trypsin microreactor for 24 h at 40 degrees c and 0. 5 ml/min. finally, the cytochrome c was digested by microreactor and the products were analysed by maldi-tof-ms. the peptide mapping for a protein by combination of the immobilized enzyme membrane microreactor and maldi-tof-ms has been developed. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; capillary zone electrophoresis ; ionization mass-spectrometry ; tryptic digest ; growth-hormone ; purification ; separation ; peptide ; phase ; antibodies |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000087161200011 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138668]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, HH,Zou, HF,Wang, HL,et al. Covalent immobilization of trypsin on glycidyl methacrylate-modified cellulose membrane as enzyme reactor[J]. chemical journal of chinese universities-chinese,2000,21(5):702-706. |
| APA | Jiang, HH,Zou, HF,Wang, HL,Ni, JY,&Zhang, Q.(2000).Covalent immobilization of trypsin on glycidyl methacrylate-modified cellulose membrane as enzyme reactor.chemical journal of chinese universities-chinese,21(5),702-706. |
| MLA | Jiang, HH,et al."Covalent immobilization of trypsin on glycidyl methacrylate-modified cellulose membrane as enzyme reactor".chemical journal of chinese universities-chinese 21.5(2000):702-706. |
入库方式： OAI收割
来源：大连化学物理研究所
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