Comparative separation of biologically active components in Rhizoma chuanxiong by affinity chromatography with alpha(1)-acid glycoprotein and human serum albumin as stationary phases
文献类型：期刊论文
| 作者 | Wang, H; Zou, H; Ni, J; Guo, B |
| 刊名 | chromatographia
 |
| 出版日期 | 2000-10-01 |
| 卷号 | 52期号:7-8页码:459-464 |
| 关键词 | column liquid chromatography alpha(1)-acid glycoprotein human serum albumin Rhizoma chuanxiong |
| 英文摘要 | affinity chromatography with alpha (1)-acid glycoprotein (agp) and human serum albumin (hsa) stationary phases was applied to screen and analyze the biologically active components of rhizoma chuanxiong. five major peaks and a number of small peaks were resolved based on their affinity for agp and hsa, respectively and three of them, identified as ferulic acid, chuanxiongzine and ligustilide via standard compounds, are regarded as effective components. the effects of acetonitrile concentration, ph, inorganic salt concentration and temperature on the retention behaviors of five major components on the two stationary phases were also investigated. it was observed that hydrophobicity is the major contributor to retention on both stationary phases, and ferulic acid has a weak electrostatic interaction with hsa. it demonstrated that the chromatograms of rhizoma chuanxiong on the two stationary phases have concise and different fingerprinting characteristics. the amount of ferulic acid, chuanxiongzine and ligustilide in rhizoma chuanxiong determined using the agp column are as much as 0.064%, 0.021% and 2.00% by weight. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | ligand-binding |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000165295100014 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138682]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA |
| 推荐引用方式 GB/T 7714 | Wang, H,Zou, H,Ni, J,et al. Comparative separation of biologically active components in Rhizoma chuanxiong by affinity chromatography with alpha(1)-acid glycoprotein and human serum albumin as stationary phases[J]. chromatographia,2000,52(7-8):459-464. |
| APA | Wang, H,Zou, H,Ni, J,&Guo, B.(2000).Comparative separation of biologically active components in Rhizoma chuanxiong by affinity chromatography with alpha(1)-acid glycoprotein and human serum albumin as stationary phases.chromatographia,52(7-8),459-464. |
| MLA | Wang, H,et al."Comparative separation of biologically active components in Rhizoma chuanxiong by affinity chromatography with alpha(1)-acid glycoprotein and human serum albumin as stationary phases".chromatographia 52.7-8(2000):459-464. |
入库方式： OAI收割
来源：大连化学物理研究所
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