Thermodynamics of porphyin binding to human serum albumin using affinity capillary electrophoresis
文献类型：期刊论文
| 作者 | Ding, YS; Lin, BC; Huie, CW |
| 刊名 | chromatographia
 |
| 出版日期 | 2000-09-01 |
| 卷号 | 52期号:5-6页码:367-370 |
| 关键词 | affinity capillary electrophoresis porphyrins human serum albumin binding constants thermodynamic parameters |
| 英文摘要 | the interaction thermodynamics of heptacarboxylporphyrin (hcp) and protoporhyrin (pp) with human serum albumin (hsa) was studied by affinity capillary electrophoresis (ace) over the temperature range of 25 - 50 degrees c, where hcp pp bound to hsa via 1:1 molecular association. the binding equilibrium constants (ph 7.4, phosphate buffer) for the binding of hcp with hsa were found to decrease wi ih an increase in temperature, whereas the binding constants of the pp/hsa system appeared to be independent of temperature changes over the range studied. the van't hoff relationship (25 - 50 degrees c) was found to be linear for the interaction of either hcp or pp with hsa. however, the interaction thermodynamics for both of these porphyrins with hsa were found to be quite different. in particular the interaction of hcp (a hydrophilic porphyrin) with hsa appeared to be based on an enthalpy-driven process, whereas the binding between pp (a hydrophobic porphyrin) and hsa driven by a favorable change in entropy. the ability of using ace to evaluate the interaction thermodynamics of serum proteins (e.g, hsa) with ligands (e.g., porphyrins and related compounds) should aid in the development of new and more effective photosensitizers in the photodynamic therapy of cancer. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000089660600019 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138769]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Hong Kong Baptist Univ, Dept Chem, Kowloon, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, YS,Lin, BC,Huie, CW. Thermodynamics of porphyin binding to human serum albumin using affinity capillary electrophoresis[J]. chromatographia,2000,52(5-6):367-370. |
| APA | Ding, YS,Lin, BC,&Huie, CW.(2000).Thermodynamics of porphyin binding to human serum albumin using affinity capillary electrophoresis.chromatographia,52(5-6),367-370. |
| MLA | Ding, YS,et al."Thermodynamics of porphyin binding to human serum albumin using affinity capillary electrophoresis".chromatographia 52.5-6(2000):367-370. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。