Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping
文献类型：期刊论文
| 作者 | Jiang, HH; Zou, HF; Wang, HL; Zhang, Q; Ni, JY; Zhang, QC; Guo, Z; Chen, XM |
| 刊名 | science in china series b-chemistry
 |
| 出版日期 | 2000-12-01 |
| 卷号 | 43期号:6页码:625-633 |
| 关键词 | immobilized enzyme microreactor glycidyl methacrylate (GMA)-modified cellulose membrane MALDI-TOF MS peptide mapping |
| 英文摘要 | matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms) has been combined with immobilized enzyme microreactor for the rapid, sensitive, and accurate tryptic mapping of protein and polypeptides. the technique utilizes the trypsin microreactor by immobilized enzyme on the glycidyl methacrylate (gma)-modified cellulose membrane. the membrane microreactor was used for the tryptic mapping of cytochrome c and the results were compared with those obtained by using free trypsin. a significant increase in the overall sensitivity of the process was observed using the membrane microreactor, as well as the elimination of background signals due to the autolysis of the trypsin. further, membrane microreactor digestions were found to be rapid and convenient. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | chromatography ; proteins |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000166664000008 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138776]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, HH,Zou, HF,Wang, HL,et al. Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping[J]. science in china series b-chemistry,2000,43(6):625-633. |
| APA | Jiang, HH.,Zou, HF.,Wang, HL.,Zhang, Q.,Ni, JY.,...&Chen, XM.(2000).Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping.science in china series b-chemistry,43(6),625-633. |
| MLA | Jiang, HH,et al."Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping".science in china series b-chemistry 43.6(2000):625-633. |
入库方式： OAI收割
来源：大连化学物理研究所
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