Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis
文献类型：期刊论文
| 作者 | Zheng, XF; Wang, JY; Wang, Y; An, LJ; Gai, HW; Hu, J |
| 刊名 | spectroscopy and spectral analysis
 |
| 出版日期 | 2001-12-01 |
| 卷号 | 21期号:6页码:804-806 |
| 关键词 | ESR spectroscopy alkaline phosphatase Cu(II) ions interaction |
| 英文摘要 | in this paper, the method of reconstitution was used to investigate the interaction between metalloenzymes (containing zn ( h)) and metal ions. electron paramagnetic resonance(epr) visible spectrum (vis) and enzyme activity assay have been employed to study the direct interactions between aminoacylase (acy) and cu( 11) ions added in aqueous solution. the results show that a dynamic equilibrium exists between the zn( h) in the active site of native enzymes and the added cu( 11) the added cu(ii) partly replaces the zn( u), forming cu( h)-enzyme derivatives. as a result, the activity of the native enzymes is influenced. in addition, the influences of ph value on this kind of interaction have also been investigated, and the results demonstrate that the decrease in the intensity of the cu ( 11) epr signal and the change of place signal in vis were observed as increase of ph value. these results suggest that the derivative of cu( it)-acy exists in solution with two different conformations, and this two conformations exchanged each other depending on ph. |
| WOS标题词 | science & technology ; technology |
| 类目[WOS] | spectroscopy |
| 研究领域[WOS] | spectroscopy |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000173061200020 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138839]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ, Coll Bioengn, Dalian 116622, Peoples R China 2.Dalian Univ Technol, Dept Bioengn, Dalian 116012, Peoples R China 3.Acad Sinica, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, XF,Wang, JY,Wang, Y,et al. Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis[J]. spectroscopy and spectral analysis,2001,21(6):804-806. |
| APA | Zheng, XF,Wang, JY,Wang, Y,An, LJ,Gai, HW,&Hu, J.(2001).Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis.spectroscopy and spectral analysis,21(6),804-806. |
| MLA | Zheng, XF,et al."Study on the direct interaction between aminoacylase and Cu(II) ions by spectroscopic analysis".spectroscopy and spectral analysis 21.6(2001):804-806. |
入库方式： OAI收割
来源：大连化学物理研究所
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