Study of isothermal adsorption of bovine gamma-globulin on nylon affinity membrane with L-tryptophane ligand by batch method
文献类型：期刊论文
| 作者 | Gan, HY; Shang, ZH; Liu, XL; Wang, JD |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2001-06-01 |
| 卷号 | 29期号:6页码:637-641 |
| 关键词 | affinity membrane chromatography L-tryptophane bovine gamma-globulin batch method |
| 英文摘要 | a new nylon affinity matrix with l-tryptophane as ligand was prepared for adsorption of bovine gamma -globulin. effects of temperature, ionic strength and ph on affinity adsorption of gamma -globulin on affinity membrane were studied by batch method. the results show that the interaction between ligand and gamma -globulin includes mainly electrostatic and hydrophobic interaction. the affinity adsorption at optimum condition obeys langmuir adsorption model with maximum adsorption capacity and minimum nonspecific adsorption. the space location between protein and ligand and the configuration of proteins will change when deviating from this condition. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | amino-acids ; chromatography ; performance ; proteins |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000169539000005 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138870]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gan, HY,Shang, ZH,Liu, XL,et al. Study of isothermal adsorption of bovine gamma-globulin on nylon affinity membrane with L-tryptophane ligand by batch method[J]. chinese journal of analytical chemistry,2001,29(6):637-641. |
| APA | Gan, HY,Shang, ZH,Liu, XL,&Wang, JD.(2001).Study of isothermal adsorption of bovine gamma-globulin on nylon affinity membrane with L-tryptophane ligand by batch method.chinese journal of analytical chemistry,29(6),637-641. |
| MLA | Gan, HY,et al."Study of isothermal adsorption of bovine gamma-globulin on nylon affinity membrane with L-tryptophane ligand by batch method".chinese journal of analytical chemistry 29.6(2001):637-641. |
入库方式： OAI收割
来源：大连化学物理研究所
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