Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate
文献类型：期刊论文
| 作者 | Ye, ML; Zou, HF; Lei, ZD; Wu, RN; Liu, Z; Ni, JY |
| 刊名 | chromatographia
 |
| 出版日期 | 2001-04-01 |
| 卷号 | 53期号:7-8页码:425-430 |
| 关键词 | capillary electrochromatography strong anion-exchange stationary phase dynamic modification SDS |
| 英文摘要 | acidic and neutral compounds have been separated by strong anion-exchange capillary electrochromatography (saxcec) dynamically modified by addition of sodium dodecylsulfate (sds). it was found that separation of neutral solutes by saxcec without addition of sds is difficult because of the weak interaction of the solutes with the hydrophilic packing surface. the hydrophobicity of the packing surface increases on addition of sds to the mobile phase, however, and the capacity of separating neutral solutes increases. acidic solutes are retained, mainly because of the ion-exchange properties of this system. the influence of mobile phase composition, e.g. sds concentration, ionic strength, and organic modifier fraction, on the retention of acidic and neutral solutes was investigated. three acids and five neutral solutes were separated in only 5 min under optimized conditions, because the direction of the strong electroosmotic flow (eof) was the some as that of electrophoretic migration of the acids under the conditions used. the repeatability of this system in terms of migration time relative standard deviation (rsd) is good - less than 0.48% for 10 consecutive runs of oil the solutes tested. column efficiencies for acids were > 125000 plates m(-1); those for neutral solutes varied from 25 000 to 100 000 plates m(-1). |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | modified cationic surfactant ; reversed-phase ; silica ; column ; electrophoresis ; peptides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168406700013 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138890]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, ML,Zou, HF,Lei, ZD,et al. Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate[J]. chromatographia,2001,53(7-8):425-430. |
| APA | Ye, ML,Zou, HF,Lei, ZD,Wu, RN,Liu, Z,&Ni, JY.(2001).Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate.chromatographia,53(7-8),425-430. |
| MLA | Ye, ML,et al."Separation of acidic and neutral compounds by strong anion-exchange capillary electrochromatography dynamically modified with sodium dodecylsulfate".chromatographia 53.7-8(2001):425-430. |
入库方式： OAI收割
来源：大连化学物理研究所
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