Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate)
文献类型：期刊论文
| 作者 | Luo, QZ; Zou, HF; Xiao, XZ; Guo, Z; Kong, L; Mao, XQ |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2001-08-17 |
| 卷号 | 926期号:2页码:255-264 |
| 关键词 | monolithic rods immobilized metal affinity chromatography protein iminodiacetic acid |
| 英文摘要 | continuous rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) was prepared by a free radical polymerization within the confines of a stainless-steel column. the epoxide groups of the rod were modified by a reaction with iminodiacetic acid (ida) that affords the active site to form metal ida chelates used for immobilized metal affinity chromatography (imac). the efficiency of coupling of ida to the epoxide-contained matrix was studied as a function of reaction time and temperature. high-performance separation of proteins, based on immobilized different metals on the column, were described. the influence of ph on the adsorption capacity of bovine serum albumin on the cu2+-ida continuous rod column was investigated in the range from 5.0 to 9.0. purification of lysozyme from egg white and human serum albumin (hsa) on the commercially available hsa solution were performed on the naked ida and cu2+-ida continuous rod columns, respectively; and the purity of the obtained fractions was detected by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. (c) 2001 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | ion affinity-chromatography ; cation-exchange chromatography ; reversed-phase chromatography ; rigid polymer monoliths ; capillary electrochromatography ; stationary-phase ; porous properties ; escherichia-coli ; continuous beds ; membrane |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170684200003 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138900]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, QZ,Zou, HF,Xiao, XZ,et al. Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate)[J]. journal of chromatography a,2001,926(2):255-264. |
| APA | Luo, QZ,Zou, HF,Xiao, XZ,Guo, Z,Kong, L,&Mao, XQ.(2001).Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate).journal of chromatography a,926(2),255-264. |
| MLA | Luo, QZ,et al."Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate)".journal of chromatography a 926.2(2001):255-264. |
入库方式： OAI收割
来源：大连化学物理研究所
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