Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography
文献类型：期刊论文
| 作者 | Luo, QZ; Zou, HF; Wang, HL; Mao, XQ; Kong, L; Ni, JY |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2001-05-01 |
| 卷号 | 29期号:5页码:497-501 |
| 关键词 | affinity chromatography polymer monolithic protein A human immunoglobulin G |
| 英文摘要 | reactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) have been prepared by "in-situ" copolymerization of the monomers in the presence of porogenic dilute, and protein a was immobilized on the continuous rods directly or through a 11 carbon atom space arm with molded synthetic methods. the properties of these two rods columns were characterized and the results showed that the medium with space arm had some extent hydrophobicity. the affinity column without space arm was used to determine the human immunoglobulin g (higg) in human serum. the correlative coefficient of the calibration curve was 0.999; the effect of the flow rate on the pressure of the continuous rods was also investigated. the subclasses of higg were separated by the use of a ph gradient. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | separation ; membrane |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000168992300001 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138929]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, QZ,Zou, HF,Wang, HL,et al. Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography[J]. chinese journal of analytical chemistry,2001,29(5):497-501. |
| APA | Luo, QZ,Zou, HF,Wang, HL,Mao, XQ,Kong, L,&Ni, JY.(2001).Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography.chinese journal of analytical chemistry,29(5),497-501. |
| MLA | Luo, QZ,et al."Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography".chinese journal of analytical chemistry 29.5(2001):497-501. |
入库方式： OAI收割
来源：大连化学物理研究所
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