An improved derivatization method for sensitive determination of fatty acids by high-performance liquid chromatography using 9-(2-hydroxylethyl)-carbazole as derivatization reagent with fluorescence detection
文献类型：期刊论文
| 作者 | You, JM; Zhang, WB; Jia, XL; Zhang, YK |
| 刊名 | chromatographia
 |
| 出版日期 | 2001-09-01 |
| 卷号 | 54期号:5-6页码:316-322 |
| 关键词 | column liquid chromatography fluorescent detection 9-(2-hydroxyethyl)-carbazole fatty acids plasma samples |
| 英文摘要 | tagging techniques with reagents used for fluorescent detection for short and long-chain fatty acids using high-performance liquid chromatography are evaluated in terms of the fagging reactions, handing, flexibility, stability of the reagents. emphasis is given to the applications of the tagging techniques to relatively high molecular mass fatty acids. the fatty acids or carboxylic compounds were derivatized to their corresponding esters with 9-(2-hydroxy ethyl)-carbazole (hec) in acetronitrile at 60 degreesc with n, n'-carbonyldiimidazole (cdi) as a coupling agent in the presence of 4-dimethylaminopyridine (dmap). a mixture of esters of c-1-c-20 fatty acids was completely separated within 45 min using gradient elution on a reversed-phase c-18 column. the maximum fluorescence emission for the derivatized fatty acids is at 365 nm (lambda (ex) 293 nm). studies on derivatization conditions indicated that fatty acids react rapidly and smoothly with hec in the presence of cdi and dmap in acetonitrile to give the corresponding sensitively fluorescent derivatives. the application of this method to the analysis of long chain fatty acids in plasma is also investigated. the lc separation shows good selectivity and reproducibility for fatty acids derivatives. the relative standard deviations (n = 6) for each fatty acid derivative are < 5.0%. the defection limits are at 38 - 57 fmol levels for c-14-c-20 fatty acids and lower levels for < c-14 fatty acids. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | carboxylic-acids ; labeling reagent ; amino-acids ; trifluoromethanesulfonate ; derivatives ; 9-anthryldiazomethane ; prostaglandins ; chloride ; serum |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000171316600004 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138965]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 2.Qufu Normal Univ, Dept Chem, Shandong 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, JM,Zhang, WB,Jia, XL,et al. An improved derivatization method for sensitive determination of fatty acids by high-performance liquid chromatography using 9-(2-hydroxylethyl)-carbazole as derivatization reagent with fluorescence detection[J]. chromatographia,2001,54(5-6):316-322. |
| APA | You, JM,Zhang, WB,Jia, XL,&Zhang, YK.(2001).An improved derivatization method for sensitive determination of fatty acids by high-performance liquid chromatography using 9-(2-hydroxylethyl)-carbazole as derivatization reagent with fluorescence detection.chromatographia,54(5-6),316-322. |
| MLA | You, JM,et al."An improved derivatization method for sensitive determination of fatty acids by high-performance liquid chromatography using 9-(2-hydroxylethyl)-carbazole as derivatization reagent with fluorescence detection".chromatographia 54.5-6(2001):316-322. |
入库方式： OAI收割
来源：大连化学物理研究所
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