Separation of basic, acidic and neutral compounds by capillary electrochromatography using uncharged monolithic capillary columns modified with anionic and cationic surfactants
文献类型：期刊论文
| 作者 | Wu, RN; Zou, HF; Ye, ML; Lei, ZD; Ni, JY |
| 刊名 | electrophoresis
 |
| 出版日期 | 2001-02-01 |
| 卷号 | 22期号:3页码:544-551 |
| 关键词 | electrochromatography dynamically modified stationary phase surfactant uncharged monolithic column |
| 英文摘要 | a mode of capillary electrochromatography (cec), based on the dynamical adsorption of surfactants on the uncharged monolithic stationary phases has been developed. the monolithic stationary phase, obtained by the in situ polymerization of butyl methacrylate with ethylene dimethacrylate, was dynamically modified with an ionic surfactant such as the long-chain quaternary ammonium salt of cetyltrimethylammonium bromide (ctab) and long-chain sodium sulfate of sodium dodecyl sulfate (sds). the ionic surfactant was adsorbed on the surface of polymeric monolith by hydrophobic interaction, and the ionic groups used to generate the electroosmotic flow (eof). the electroosmotic mobility through these capillary columns increased with increasing the content of ionic surfactants in the mobile phase. in this way, the synthesis of the monolithic stationary phase with binary monomers can be controlled more easily than that with ternary monomers, one of which should be an ionic monomer to generate eof. furthermore, it is more convenient to change the direction and magnitude of eof by changing the concentration of cationic or anionic surfactants in this system. an efficiency of monolithic capillary columns with more than 140 000 plates per meter for neutral compounds has been obtained, and the relative standard deviations observed for t(0) and retention factors of neutral solutes were about 0.22% and less than 0.56% for ten consecutive runs, respectively. effects of mobile phase composition on the eof of the column and the retention values of the neutral solutes were investigated. simultaneous separation of basic, neutral and acidic compounds has been achieved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | rigid polymer monoliths ; performance liquid-chromatography ; dynamically modified silica ; electroosmotic flow ; zone electrophoresis ; stationary phases ; ion-exchange ; selectivity ; media ; bases |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167362400026 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/138995]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, RN,Zou, HF,Ye, ML,et al. Separation of basic, acidic and neutral compounds by capillary electrochromatography using uncharged monolithic capillary columns modified with anionic and cationic surfactants[J]. electrophoresis,2001,22(3):544-551. |
| APA | Wu, RN,Zou, HF,Ye, ML,Lei, ZD,&Ni, JY.(2001).Separation of basic, acidic and neutral compounds by capillary electrochromatography using uncharged monolithic capillary columns modified with anionic and cationic surfactants.electrophoresis,22(3),544-551. |
| MLA | Wu, RN,et al."Separation of basic, acidic and neutral compounds by capillary electrochromatography using uncharged monolithic capillary columns modified with anionic and cationic surfactants".electrophoresis 22.3(2001):544-551. |
入库方式： OAI收割
来源：大连化学物理研究所
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