Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor
文献类型：期刊论文
| 作者 | Guo, Z; Zhang, QC; Lei, ZD; Kong, L; Mao, XQ; Zou, HF |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2002-07-01 |
| 卷号 | 23期号:7页码:1277-1280 |
| 关键词 | nano-liter micro-reactor capillary immobilized enzyme MALDI-TOF-MS peptide mapping analysis |
| 英文摘要 | peptide mapping analysis by combining the enzymatic digestion and the maldi-tof-ms has emerged as a powerful tool for identification and characterization of protein. we describe a nanoliter microreactor prepared with immobilization of enzyme on the porous wall of a capillary. the advantages of the prepared micro-reactor for digestion of the proteins are the elimination of enzyme autolysis and concentration of the micro-sample. horse heart cytochrome c is selected as the target protein for the evaluation of micro-reactor, and it is found that about 0.1 picomole of protein analyte could routinely be digested for peptide mapping analysis and even femtomole of protein can be well characterized. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | electrochromatography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000176908400015 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139061]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&S Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Z,Zhang, QC,Lei, ZD,et al. Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor[J]. chemical journal of chinese universities-chinese,2002,23(7):1277-1280. |
| APA | Guo, Z,Zhang, QC,Lei, ZD,Kong, L,Mao, XQ,&Zou, HF.(2002).Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor.chemical journal of chinese universities-chinese,23(7),1277-1280. |
| MLA | Guo, Z,et al."Studies on rapid micro-scale peptide mapping analysis using a capillary micro-reactor".chemical journal of chinese universities-chinese 23.7(2002):1277-1280. |
入库方式： OAI收割
来源：大连化学物理研究所
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