High-performance affinity chromatography with immobilization of protein A and L-histidine on molded monolith
文献类型：期刊论文
| 作者 | Luo, QZ; Zou, HF; Zhang, Q; Xiao, XZ; Ni, JY |
| 刊名 | biotechnology and bioengineering
 |
| 出版日期 | 2002-12-05 |
| 卷号 | 80期号:5页码:481-489 |
| 关键词 | affinity chromatography monolith protein A L-histidine human IgG |
| 英文摘要 | reactive monoliths of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) have been prepared by "in-situ" copolymerization of the monomers in the presence of porogenic diluents. protein a and l-histidine were immobilized on the monoliths directly or through a spacer arm, respectively. the properties of these two kinds of affinity columns were characterized, and the results showed that the columns with coupling of ligands by a spacer arm have some extent of non-specific adsorption for bovine serum albumin. the affinity column based on the monolithic polymer support provided us with good hydrodynamic characteristic, low flow resistance, and easy preparation. these two affinity columns were used for the purification of immunoglobulin g from human serum. the purity of the purified igg was detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof-ms). the stability of the protein a affinity column was investigated, and its performance remained invariable after half a year. the effects of the nature and the ph of the buffer system on the adsorption capacity of human igg on histidyl affinity column were also investigated. the protein a affinity column is favorable for rapid analysis of human igg samples. in contrast, the advantages of mild elution conditions, high stability, as well as low cost provide the histidyl column further potential possibility for fast removal of igg from human plasma in clinical applications. (c) 2002 wiley periodicals, inc. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | hollow-fiber membranes ; reversed-phase chromatography ; co-ethylene dimethacrylate) ; immunoglobulin-g ; exchange chromatography ; pseudobioaffinity chromatography ; adsorption capacity ; stationary-phase ; separation ; purification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000178822100001 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139081]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, QZ,Zou, HF,Zhang, Q,et al. High-performance affinity chromatography with immobilization of protein A and L-histidine on molded monolith[J]. biotechnology and bioengineering,2002,80(5):481-489. |
| APA | Luo, QZ,Zou, HF,Zhang, Q,Xiao, XZ,&Ni, JY.(2002).High-performance affinity chromatography with immobilization of protein A and L-histidine on molded monolith.biotechnology and bioengineering,80(5),481-489. |
| MLA | Luo, QZ,et al."High-performance affinity chromatography with immobilization of protein A and L-histidine on molded monolith".biotechnology and bioengineering 80.5(2002):481-489. |
入库方式： OAI收割
来源：大连化学物理研究所
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