Preparation and characterization of protein A immobilized on monolithic capillary column for affinity chromatography
文献类型：期刊论文
| 作者 | Pan, ZF; Mo, WM; Wu, RA; Luo, QZ; Ye, ML; Zou, HF |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2002-11-01 |
| 卷号 | 23期号:11页码:2070-2072 |
| 关键词 | affinity chromatography monolithic capillary column protein A human immunoglobulin G |
| 英文摘要 | an assay technique for the determination of the human igg in human plasma has been developed by utilizing. protein a immobilized monolithic capillary column. the affinity chromatography experiment was performed on a capillary electrophoresis instrument by using its pressure system as the driving force. reactive monolithic capillary columns have been prepared, by in-situ copolymerization of the monomers in the presence of porogenic dilute, and protein a was immobilized on the monolithic rods directly or;through a 11 carbon atom space i arm with molded synthetic methods. the non-specific adsorption of two kinds of media has been studied and-the results showed that the medium without space arm gives very low non-specific adsorption of bsa. the affinity column without space arm was used to determine the human immunoglobulin g (higg) in human serums the correlative. coefficient of the calibration curve reaches 0.998 7. the total time of rapid analysis was less than 0.7 min. the consumption of eluent buffer and sample is much low than by conventional hplc. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | immunoaffinity chromatography ; immunoglobulin-g ; biomolecules ; purification ; separation ; polymers ; supports |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000179416200015 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139096]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Zhejiang Univ Technol, Coll Chem Engn, Hangzhou 310014, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, ZF,Mo, WM,Wu, RA,et al. Preparation and characterization of protein A immobilized on monolithic capillary column for affinity chromatography[J]. chemical journal of chinese universities-chinese,2002,23(11):2070-2072. |
| APA | Pan, ZF,Mo, WM,Wu, RA,Luo, QZ,Ye, ML,&Zou, HF.(2002).Preparation and characterization of protein A immobilized on monolithic capillary column for affinity chromatography.chemical journal of chinese universities-chinese,23(11),2070-2072. |
| MLA | Pan, ZF,et al."Preparation and characterization of protein A immobilized on monolithic capillary column for affinity chromatography".chemical journal of chinese universities-chinese 23.11(2002):2070-2072. |
入库方式： OAI收割
来源：大连化学物理研究所
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