Preparation and chromatographic behavior of acetate-fiber filter rods with dye affinity ligands
文献类型：期刊论文
| 作者 | Li, JH; Wang, JD; Liu, XL |
| 刊名 | chromatographia
 |
| 出版日期 | 2002-10-01 |
| 卷号 | 56期号:7-8页码:401-406 |
| 关键词 | affinity chromatography acetate fiber filter rod cibacron blue F3GA and reactive red 120 albumin |
| 英文摘要 | the dyes cibacron blue f3ga and reactive red 120 have been immobilized on acetate fiber filter rods to produce potential affinity matrixes. the isothermal adsorption of bovine serum albumin or human serum albumin on these matrixes was investigated and proved to conform to the freundlich equation. in the static adsorption of human plasma the adsorption capacity for human serum albumin was 12.5 mg g(-1) fiber filter and one bond was observed in sds-page electrophoresis of human serum albumin isolated by the immobilized cibacron blue f3ga filter rod. ligand utilization efficiencies and breakthrough volumes were obtained for adsorption of hsa on the cibacron blue f3ga filter rod when the feed-rates were from 0.5 to 6 ml min(-1). in the affinity chromatography of human plasma the yield of human serum albumin was 1.27 g per 100 ml plasma. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | purification ; proteins ; design |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000178836600002 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139121]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, JH,Wang, JD,Liu, XL. Preparation and chromatographic behavior of acetate-fiber filter rods with dye affinity ligands[J]. chromatographia,2002,56(7-8):401-406. |
| APA | Li, JH,Wang, JD,&Liu, XL.(2002).Preparation and chromatographic behavior of acetate-fiber filter rods with dye affinity ligands.chromatographia,56(7-8),401-406. |
| MLA | Li, JH,et al."Preparation and chromatographic behavior of acetate-fiber filter rods with dye affinity ligands".chromatographia 56.7-8(2002):401-406. |
入库方式： OAI收割
来源：大连化学物理研究所
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