Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)
文献类型：期刊论文
| 作者 | You, JM; Zhang, YK |
| 刊名 | chromatographia
 |
| 出版日期 | 2002-07-01 |
| 卷号 | 56期号:1-2页码:43-50 |
| 关键词 | column liquid chromatography fluorescent detection 2-(9-carbazole)ethyl chloroformate biogenic amines |
| 英文摘要 | the use of 2-(9-carbazole)ethyl chloroformate (ceoc) for pre-column derivatization of biogenic amines (ba) has been tested for the first time. the reagent reacts completely with ba within 3 min at ambient temperature in acetonitrile solution to form stable derivatives that are readily analyzed by reversed-phase hplc. study of the derivatization conditions revealed derivatization yields to be excellent in borate buffer over the ph range 9.0- 10.0. maximum yields were obtained by use of a three- to fourfold molar excess of reagent. the reaction is extremely tolerant of common buffer salts, no decrease in reaction yield is discernible in well-buffered samples. the emission maximum for the ceoc-derivatives is 360 nm (lambda(ex) = 293 nm). all the derivatives fluoresced strongly and direct injection of the reaction mixture was possible, with no significant disturbance from the major fluorescent reagent degradation by-products, 2-(9-carbazole)ethanol (ceoc-oh) and bis-(2-(9-carbazole)ethyl) carbonate (ceoc)(2). separation of the derivatized ba by high-performance liquid chromatography with gradient elution was tested on a hypersil bds c18 column. excellent response linearity was observed over the concentration range from 0.25 to 94.6 mumol l-1 for the labeled ba. detection limits were 117 - 840 fmol at a signal-to-noise ratio of 3: 1. analysis of ba in a shrimp sauce extract was conducted to demonstrate the applicability of the technique to real sample matrixes; results were satisfactory. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | liquid-chromatographic determination ; automated precolumn derivatization ; rp-hplc ; o-phthaldialdehyde ; chloride ; acids ; foods ; fish ; hydroxyproline ; isothiocyanate |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000177132900008 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139175]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 2.Qufu Normal Univ, Dept Chem, Qufu 273165, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, JM,Zhang, YK. Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)[J]. chromatographia,2002,56(1-2):43-50. |
| APA | You, JM,&Zhang, YK.(2002).Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC).chromatographia,56(1-2),43-50. |
| MLA | You, JM,et al."Determination of biogenic Amines by RPHPLC with fluorescent detection after derivatization with 2-(9-carbazole)ethyl chloroformate (CEOC)".chromatographia 56.1-2(2002):43-50. |
入库方式： OAI收割
来源：大连化学物理研究所
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