Determination of Amines by high performance liquid chromatography with a pre-column derivatization and fluorescence detection
文献类型：期刊论文
| 作者 | Zhang, L; Ping, GC; You, JM; Ming, YF; Zhang, WB; Zhang, YK |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2003-07-01 |
| 卷号 | 31期号:7页码:808-811 |
| 关键词 | chloroformate fluorescence reagent fluorescence derivatization high performance liquid chromatography amines |
| 英文摘要 | 2-(9-carbazole) ethyl chloroformate (ceoc), a novel fluorescence derivatization reagent, has been firstly developed for the determiantion of amines. in a one-step procedure, ceoc reacts with the amines to form stable derivatives. maximum yield is observed with a molar reagent excess of approximately 3 to 4 times for each of the amines. the method in conjunction with a gradient elution offers a baseline separation on a reversed-phase hypersil bds c-18 column. mobile phase: a = acetonitrile/water (20/80, v/v); b = acetonitrile/water (95/5, v/v) flow rate = 1.0 ml/min; column temperature was set at 35 degreesc. the fluorescent derivatives are detected at lambda(ex)/lambda(em) = 293/365 nm. it was proved that ceoc-high performance liquid chromatography (hplc) not only had excellent precision and accuracy but also high tolerance of the matrix of samples (including salt), therefore the method is suitable for the determination of biological materials, foods and feeds. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate ; acids |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184609900009 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139252]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 2.Qufu Normal Univ, Dept Chem, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, L,Ping, GC,You, JM,et al. Determination of Amines by high performance liquid chromatography with a pre-column derivatization and fluorescence detection[J]. chinese journal of analytical chemistry,2003,31(7):808-811. |
| APA | Zhang, L,Ping, GC,You, JM,Ming, YF,Zhang, WB,&Zhang, YK.(2003).Determination of Amines by high performance liquid chromatography with a pre-column derivatization and fluorescence detection.chinese journal of analytical chemistry,31(7),808-811. |
| MLA | Zhang, L,et al."Determination of Amines by high performance liquid chromatography with a pre-column derivatization and fluorescence detection".chinese journal of analytical chemistry 31.7(2003):808-811. |
入库方式： OAI收割
来源：大连化学物理研究所
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