A simple and robust set-up for on-column sample preconcentration - nano-liquid chromatography - electrospray ionization mass spectrometry for the analysis of N-acylhomoserine lactones
文献类型：期刊论文
| 作者 | Frommberger, M; Schmitt-Kopplin, P; Ping, G; Frisch, H; Schmid, M; Zhang, Y; Hartmann, A; Kettrup, A |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2004-02-01 |
| 卷号 | 378期号:4页码:1014-1020 |
| 关键词 | quorum sensing AHL nano-HPLC sample preconcentration mass spectrometry |
| 英文摘要 | a simple method for the simultaneous, rapid and sensitive determination of n-acylhomoserine lactone signaling molecules in bacterial isolates, without prior sample preconcentration and with minimal sample cleanup, is presented. the analysis relies on the combination of analyte preconcentration and separation on a single device: a relatively large sample volume (1-5 mul) is directly loaded onto a laboratory-made, miniaturized (75 mum i.d.) reverse phase nano-liquid chromatography column, connected on-line to a microelectrospray-ionization ion trap mass spectrometer. in a first step the analyte is adsorbed (and so concentrated) at the beginning of the column, and is eluted and selectively separated in a second step by the organic mobile phase. sample preconcentration follows the mechanisms of solid phase extraction on a nano-scale, while separation takes place according to classical liquid chromatography separation principles. the columns can be manufactured easily, are simply connected, and used with minimal solvent amounts; this makes this method extremely robust and cost-effective. the analytical setup was found to be routinely quantitative down to a concentration of 10 ng/ml (corresponding to a total analyte amount of 10 pg or ca. 50 fmol). the limit of detection was reached at 1 ng/ml (1 pg, ca. 5 fmol). compared to the classical ahl analysis of bacterial cultures with biosensors, where selectivity and sensitivity is often limited, this rapid analytical technique is a substantial qualitative and quantitative improvement. two unsubstituted n-acylhomoserine lactones could be identified and quantified from a burkholderia cepacia culture supernatant in a chloroform extract. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | cell-cell communication ; large-volume injection ; l-homoserine lactones ; pseudomonas-aeruginosa ; burkholderia-cepacia ; structural identification ; capillary electrochromatography ; interspecies communication ; staphylococcus-aureus ; autoinducer |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000189194800020 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139306]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.GSF Natl Res Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany 2.GSF Natl Res Ctr Environm & Hlth, Inst Soil Ecol, Rhizosphere Biol Grp, D-85764 Neuherberg, Germany 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 4.Tech Univ Munich, Lehrstuhl Okol Chem & Umweltanalyt, D-8050 Freising Weihenstephan, Germany |
| 推荐引用方式 GB/T 7714 | Frommberger, M,Schmitt-Kopplin, P,Ping, G,et al. A simple and robust set-up for on-column sample preconcentration - nano-liquid chromatography - electrospray ionization mass spectrometry for the analysis of N-acylhomoserine lactones[J]. analytical and bioanalytical chemistry,2004,378(4):1014-1020. |
| APA | Frommberger, M.,Schmitt-Kopplin, P.,Ping, G.,Frisch, H.,Schmid, M.,...&Kettrup, A.(2004).A simple and robust set-up for on-column sample preconcentration - nano-liquid chromatography - electrospray ionization mass spectrometry for the analysis of N-acylhomoserine lactones.analytical and bioanalytical chemistry,378(4),1014-1020. |
| MLA | Frommberger, M,et al."A simple and robust set-up for on-column sample preconcentration - nano-liquid chromatography - electrospray ionization mass spectrometry for the analysis of N-acylhomoserine lactones".analytical and bioanalytical chemistry 378.4(2004):1014-1020. |
入库方式： OAI收割
来源：大连化学物理研究所
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