Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography
文献类型：期刊论文
| 作者 | Fu, HJ; Jin, WH; Xiao, H; Xie, CH; Guo, BC; Zou, HF |
| 刊名 | electrophoresis
 |
| 出版日期 | 2004-02-01 |
| 卷号 | 25期号:4-5页码:600-606 |
| 关键词 | basic pharmaceuticals capillary electrochromatography human serum hydrophilic interaction strong-cation exchange |
| 英文摘要 | hydrophilic interaction capillary electrochromatography (hi-cec) for the determination of basic pharmaceuticals spiked in human serum is described. the organic modifier content, ionic strength, and ph value of the mobile phase as well as the applied voltage are optimized for separation and elution of these drug analytes. excellent separation was achieved for drugs using a mobile phase composition of 80% v/v acetonitrile in 100 mm triethylamine phosphate (teap) buffer at ph 2.8 with column efficiencies for analytes more than 200 000 plates/m. the samples of human serum spiked with basic drugs were directly injected after a simple acetonitrile treatment. the linear range and reproducibility of these basic drugs using an external and internal standard method were compared. as a result, the reproducibility could be greatly improved by using the internal standard method. good calibration curves with regression coefficients more than 0.998 in the range of 5-160 mug/ml were observed with the internal standard method. the limits of quantitation, based on standards with acceptable relative standard deviations (rsds), were below 5 mug/ml. the intra- and inter-day precisions, determined as rsds, were less than 4.57%. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; reversed-phase ; stationary phases ; polar compounds ; separation ; electrophoresis ; silica ; drugs ; ibuprofen ; behavior |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220046200009 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139327]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, HJ,Jin, WH,Xiao, H,et al. Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography[J]. electrophoresis,2004,25(4-5):600-606. |
| APA | Fu, HJ,Jin, WH,Xiao, H,Xie, CH,Guo, BC,&Zou, HF.(2004).Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography.electrophoresis,25(4-5),600-606. |
| MLA | Fu, HJ,et al."Determination of basic pharmaceuticals in human serum by hydrophilic interaction capillary electrochromatography".electrophoresis 25.4-5(2004):600-606. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。