Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis
文献类型：期刊论文
| 作者 | Wang, C; Zheng, YF; Xiong, JH; Weng, QF; Yang, J; Yang, Q; Xu, GW |
| 刊名 | acta chimica sinica
 |
| 出版日期 | 2004-02-14 |
| 卷号 | 62期号:3页码:279-282 |
| 关键词 | affinity capillary electrophoresis human alpha-lactalbumin calcium binding |
| 英文摘要 | affinity capillary electrophoresis was used to study the interaction of human alpha-lactalbumin (alpha-hla) with calcium ion(ca2+). with constant concentration of alpha-hla as a receptor and various concentrations of ca2+ as a ligand in the running buffer, changes in electrophoretic mobilities of alpha-hla were observed when complexes of alpha-hla and ca2+ came into being. based on scatchard analysis of the mobility ratios (m) of alpha-hla to ca2+, it was found that the apparent binding constant (k-app) was 2.0 x 10(7) (mol/l)(-1). in addition, the effect of ca2+ on the unfolding of alpha-hla induced by urea and heating was studied, and the results showed that the binding of ca2+ increased the stability of alpha-hla against the action of denaturing agent (such as urea) and heating. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | biomolecular recognition ; binding constants ; bovine ; protein |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000188877700010 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139365]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, C,Zheng, YF,Xiong, JH,et al. Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis[J]. acta chimica sinica,2004,62(3):279-282. |
| APA | Wang, C.,Zheng, YF.,Xiong, JH.,Weng, QF.,Yang, J.,...&Xu, GW.(2004).Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis.acta chimica sinica,62(3),279-282. |
| MLA | Wang, C,et al."Study on human alpha-lactalbumin-calcium interaction using affinity capillary electrophoresis".acta chimica sinica 62.3(2004):279-282. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。