Construction of two-dimensional separation platform of coupling capillary isoelectric focusing with capillary non-gel sieving electrophoresis for proteins
文献类型：期刊论文
| 作者 | Liu, HC; Yang, C; Yang, Q; Zhang, WB; Zhang, YK |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2004-03-01 |
| 卷号 | 32期号:3页码:273-277 |
| 关键词 | hollow fiber interface two-dimensional separation platform capillary isoelectric focusing electrophoresis capillary non-gel sieving electrophoresis |
| 英文摘要 | a novel dialysis hollow fiber interface was designed and fabricated and a two-dimensional separation platform of coupling capillary isoelectric focusing (cief) with capillary non-gel sieving electrophoresis (cnge) for proteins was constructed by the interface. classic two-dimensional polyacrylamide gel electrophoresis (2-d page) was transferred to the capillary format via the interface. the hemoglobin (hb) sample was selected to demonstrate the 2-d separation system's feasibility and resolving power. the 2-d separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | chromatography ; proteomics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220700500001 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139383]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, HC,Yang, C,Yang, Q,et al. Construction of two-dimensional separation platform of coupling capillary isoelectric focusing with capillary non-gel sieving electrophoresis for proteins[J]. chinese journal of analytical chemistry,2004,32(3):273-277. |
| APA | Liu, HC,Yang, C,Yang, Q,Zhang, WB,&Zhang, YK.(2004).Construction of two-dimensional separation platform of coupling capillary isoelectric focusing with capillary non-gel sieving electrophoresis for proteins.chinese journal of analytical chemistry,32(3),273-277. |
| MLA | Liu, HC,et al."Construction of two-dimensional separation platform of coupling capillary isoelectric focusing with capillary non-gel sieving electrophoresis for proteins".chinese journal of analytical chemistry 32.3(2004):273-277. |
入库方式： OAI收割
来源：大连化学物理研究所
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