Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the p53 gene
文献类型：期刊论文
| 作者 | Zhao, CX; Xu, GW; Shi, XZ; Ma, JM; Zhang, Y; Lv, S; Weng, QF; Yang, Q |
| 刊名 | analytical sciences
 |
| 出版日期 | 2004-07-01 |
| 卷号 | 20期号:7页码:1001-1005 |
| 英文摘要 | fluorescent-based single-strand conformation polymorphism (f-sscp) analysis with capillary electrophoresis (ce) is the most common method for the detection of mutation because of its high sensitivity and resolution. in this study, we prepared an inexpensive linear polyacrylamide (lpa), and successfully applied it to ce-sscp analysis and tandem cesscp/heteroduplex analysis (ha) of the p53 gene on an abi capillary genetic analyzer. a comparison of the sieving capabilities of a homemade lpa and commercial polydimethylacrylamide (pdma) demonstrates that the homemade lpa has a higher resolution, a shorter analysis time, and is more suitable for tandem sscp/ha than commercial pdma. to show the usefulness, mutations of p53 gene exon 7 - 8 in 37 tumor samples were investigated by using homemade lpa. the results indicate that 10 mutations were found in 9 of 37 cases; the majority of p53 mutations were missense mutations, and 70% were located in exon 7, which plays an important role in neoplastic progression in human tumorigenesis. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | polymorphism analysis ; polymer-solutions ; high-throughput ; dna ; separation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222697500002 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139487]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromtog R&A, Dalian 116011, Peoples R China 2.Dalian Med Univ, Secondary Affiliated Hosp, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, CX,Xu, GW,Shi, XZ,et al. Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the p53 gene[J]. analytical sciences,2004,20(7):1001-1005. |
| APA | Zhao, CX.,Xu, GW.,Shi, XZ.,Ma, JM.,Zhang, Y.,...&Yang, Q.(2004).Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the p53 gene.analytical sciences,20(7),1001-1005. |
| MLA | Zhao, CX,et al."Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the p53 gene".analytical sciences 20.7(2004):1001-1005. |
入库方式： OAI收割
来源：大连化学物理研究所
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