Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes
文献类型：期刊论文
| 作者 | Hao, WQ; Wang, JD; Li, JH |
| 刊名 | chromatographia
 |
| 出版日期 | 2004-10-01 |
| 卷号 | 60期号:7-8页码:449-454 |
| 关键词 | membrane-affinity chromatography frontal analysis cibacron blue F3GA bovine serum albumin |
| 英文摘要 | the adsorption of bovine serum albumin (bsa) on cibacron blue f3ga cellulose affinity membranes has been studied by frontal analysis. the ideal stirred tank (ist) model, the modified stirred tank (mst) model, and the equilibrium-dispersive (e-d) model were used to describe experimental breakthrough curves. the e-d model was found to best describe the initial region of the breakthrough curve whereas the mst model was best for the late region, implying a difference between the initial and late stages of adsorption. according to the e-d model, the effect of axial dispersion was not negligible, possibly because of experimental factors such as inhomogeneous distribution of the feed solution and the thinness of affinity membranes. the variable a of the mst model can be taken as the adjusted number of theoretical plates of the affinity membranes. the maximum value of a was found experimentally by varying the flow rate; increasing the feed concentration led to a smaller value of alpha. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | affinity-membrane ; sorption kinetics ; chromatography ; bioseparations ; proteins ; breakthrough ; purification ; design |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224907300013 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139496]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, WQ,Wang, JD,Li, JH. Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes[J]. chromatographia,2004,60(7-8):449-454. |
| APA | Hao, WQ,Wang, JD,&Li, JH.(2004).Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes.chromatographia,60(7-8),449-454. |
| MLA | Hao, WQ,et al."Modeling, by frontal analysis, of the adsorption of bovine serum albumin on cibacron blue-modified cellulose membranes".chromatographia 60.7-8(2004):449-454. |
入库方式： OAI收割
来源：大连化学物理研究所
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