Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography
文献类型：期刊论文
| 作者 | Ma, XQ; Wang, LX; Xu, Q; Zhang, F; Xiao, HB; Liang, XM |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2004-02-01 |
| 卷号 | 25期号:2页码:238-242 |
| 关键词 | high performance liquid chromatography(HPLC) retention equation gradient optimization ginsenoside |
| 英文摘要 | a multi-segment gradient method for the separation of ginsenosides in reversed phase-high performance liquid chromatography (rp-hplc) was developed. the retention parameters of 8 ginsenosides (rg1, re, rf, rg2, rb1, rc, rb2 and rd) were obtained on an ods2 c18 column (4.6 mm x 250 mm) from a series of isocratic acetonitrile-water elution. it is found that two-parameter retention equation of ginsenosides can't be used to accurately predict the resolution, but the three-parameter retention equation can. the reason is that the retention factor k' decreases fast while the volume fractions of acetonitrile increase in mobile phase, which make the precision of two-parameter retention equation can't satisfy the predicting need of resolution. based on the three-parameter retention equation, an optimum elution program consisting of three segments was developed. under the optimized gradient conditions, the retention time and resolution of ginsenosides are in good agreement with the predicted ones. the analysis time of ginsenosides is shortened and the resolution is improved by the method. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | elution |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000189187400010 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139601]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, XQ,Wang, LX,Xu, Q,et al. Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography[J]. chemical journal of chinese universities-chinese,2004,25(2):238-242. |
| APA | Ma, XQ,Wang, LX,Xu, Q,Zhang, F,Xiao, HB,&Liang, XM.(2004).Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography.chemical journal of chinese universities-chinese,25(2),238-242. |
| MLA | Ma, XQ,et al."Optimization method of multi-segment gradient for separation of ginsenosides in reversed phase-high performance liquid chromatography".chemical journal of chinese universities-chinese 25.2(2004):238-242. |
入库方式： OAI收割
来源：大连化学物理研究所
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