Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles
文献类型：期刊论文
| 作者 | Ye, ZQ; Tan, MQ; Wang, GL; Yuan, JL |
| 刊名 | journal of fluorescence
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 15期号:4页码:499-505 |
| 关键词 | terbium complex fluorescent nanoparticle fluorescence probe time-resolved fluoroimmunoassay |
| 英文摘要 | novel zirconia-based fluorescent terbium nanoparticles have been prepared as a fluorescent nanoprobe for time-resolved fluorescence bioassay. the nanoparticles were prepared in a water-in-oil (w/o) microemulsion consisting of a strongly fluorescent tb3+ complex, n, n, n-1, n-1-[2,6- bis(3'-aminomethyl-1 - pyrazolyl)- phenylpyridine] tetrakis( acetate)- tb3+(bpta-tb3+), triton x-100, hexanol, and cyclohexane by controlling co-condensation of zr(och2ch3)(4) and zrocl2. the characterizations by transmission electron microscopy and fluorometric methods indicate that the nanoparticles are uniform in size, 33 +/- 4 nm in diameter, and have a fluorescence quantum yield of 8.9% and a long fluorescence lifetime of 2.0 ms. the zirconia-based fluorescent terbium nanoparticles show high stability against basic dissolution in a high ph aqueous buffer compared to the silica-based nanoparticles. a surface modification and bioconjugation method for the fluorescent nanoparticles was developed, and the nanoparticle-conjugated streptavidin (sa) was used for time-resolved floroimmunoassy (tr-fia) of human prostate specific antigen (psa). the result shows that the zirconia-based fluorescent terbium nanoparticles are useful as a fluorescent nanoprobe for time-resolved fluorescence bioassay. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | time-resolved fluoroimmunoassay ; performance liquid-chromatography ; modified porous zirconia ; bioconjugated nanoparticles ; fluorometric application ; europium nanoparticles ; amino-acids ; proteins ; silica ; temperature |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231940700006 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139646]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, ZQ,Tan, MQ,Wang, GL,et al. Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles[J]. journal of fluorescence,2005,15(4):499-505. |
| APA | Ye, ZQ,Tan, MQ,Wang, GL,&Yuan, JL.(2005).Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles.journal of fluorescence,15(4),499-505. |
| MLA | Ye, ZQ,et al."Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles".journal of fluorescence 15.4(2005):499-505. |
入库方式： OAI收割
来源：大连化学物理研究所
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