Microarrays of DNA and protein integrated on microfluidic chip
文献类型：期刊论文
| 作者 | Zhang, ZX; Shen, Z; Zhao, H; Li, B; Song, SP; Hu, J; Lin, BC; Li, MQ |
| 刊名 | acta chimica sinica
 |
| 出版日期 | 2005-09-28 |
| 卷号 | 63期号:18页码:1743-1746 |
| 关键词 | microarray microfluidic chip modification polylysine |
| 英文摘要 | to combine the advantages of high-throughput microarray and microfluidic chip technology, microarray was fabricated in channels. dna and protein were arrayed on quartz slide, which was reversibly sealed by poly(dimethylsiloxane) (pdms) slab containing pattern of channels. the hybridization carried out in channels indicated that target dna and proteins were bound to their complementary dna and protein probes specifically. in this method, pdms conjugated with polylysine was contributed to imparting the wettability and decreasing unspecific adsorption of biomolecules. our data demonstrated that the integrated chip speeded up the detection compared to microarrays, extended the parallel channels with serial properties and enhanced the throughput of probes and targets. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | gene-expression ; device ; arrays ; probes ; flow |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000232089800020 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139656]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China 4.Shanghai Jiao Tong Univ, Nanobiol Lab, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, ZX,Shen, Z,Zhao, H,et al. Microarrays of DNA and protein integrated on microfluidic chip[J]. acta chimica sinica,2005,63(18):1743-1746. |
| APA | Zhang, ZX.,Shen, Z.,Zhao, H.,Li, B.,Song, SP.,...&Li, MQ.(2005).Microarrays of DNA and protein integrated on microfluidic chip.acta chimica sinica,63(18),1743-1746. |
| MLA | Zhang, ZX,et al."Microarrays of DNA and protein integrated on microfluidic chip".acta chimica sinica 63.18(2005):1743-1746. |
入库方式： OAI收割
来源：大连化学物理研究所
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