Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum
文献类型：期刊论文
| 作者 | Wang, ZC; Zhang, QG; Zhang, WB; Li, T; Zhao, ZY |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2005-04-01 |
| 卷号 | 33期号:4页码:437-441 |
| 关键词 | comprehensive two-dimensional liquid chromatography protein peak capacity goat serum |
| 英文摘要 | the system constructed here is a comprehensive two-dimensional liquid chromatography that uses gel filtration chromatography ( gfc) followed by reversed phase liquid chromatography ( rp). column of shodex protein kw-802. 5 (300 mm x 8 mm i. d.) acts as the first dimension which was eluted with 0. 2 mol/l nah2po4 ( ph 7. 0) at a flow rate of 0. 15 ml/min. the second dimension uses the column of hypersil bds c-18 (35 mm x 4. 6 mm i. d.) which was operated in gradient form. at a flow rate of 3. 0 ml/min. the interface is a 2-position-10-port valve coupled with two parallel columns which analyzed elutes from gfc alternated. the system was evaluated by 5 standard proteins that cannot be-resolved in either gfc or rp mode alone but can be resolved by the comprehensive two-dimensional liquid chromatography. the total peak capacity of this system is calculated to be 225. compared with one-dimensional chromatography, the total peak capacity and the resolution are enhanced. the system was used for separating goat serum to validate and isolate the overlap ingredients. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | statistical-theory ; peptides ; proteins ; instrumentation ; overlap ; system |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228870200001 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139679]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Elite Analyt Instruments Co Ltd, Dalian 116011, Peoples R China 2.China Univ Geosci, Fac Chem Engn & Mat Sci, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, ZC,Zhang, QG,Zhang, WB,et al. Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum[J]. chinese journal of analytical chemistry,2005,33(4):437-441. |
| APA | Wang, ZC,Zhang, QG,Zhang, WB,Li, T,&Zhao, ZY.(2005).Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum.chinese journal of analytical chemistry,33(4),437-441. |
| MLA | Wang, ZC,et al."Construction of comprehensive two-dimensional liquid chromatography for the analysis of goat serum".chinese journal of analytical chemistry 33.4(2005):437-441. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。