Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry
文献类型：期刊论文
| 作者 | Yang, J; Xu, GW; Zheng, WF; Kong, HW; Wang, C; Zhao, XJ; Pang, T |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2005-08-19 |
| 卷号 | 1084期号:1-2页码:214-221 |
| 关键词 | metabonomics peak alignment liver cancer pattern recognition |
| 英文摘要 | metabonomics, the study of metabolites and their roles in various disease states, is a novel methodology arising from the post-genomics era. this methodology has been applied in many fields. current metabonomic practice has relied on mass spectrometry (ms), gas chromatography-mass spectrometry (gc-ms), and nuclear magnetic resonance (nmr) to analyze metabolites. in this study, a strategy was developed for applying high-performance liquid chromatography (hplc) and lc-ms-ms to metabonomics research. one of the key problems to be solved in this strategy is to match the peaks between the chromatograms. a peak alignment algorithm has been developed to match the chromatograms before the pattern recognition. as an application example, the strategy described above was applied to metabonomics research on liver diseases, and the false-positive result of live cancer diagnosis from the hepatocirrhosis and hepatitis diseases was effectively reduced to 7.40%. based on the pattern recognition, several potential biomarkers were found and further identified by the following lc-ms-ms experiments. the structures of eight potential biomarkers were given for distinguishing the liver cancer from the hepatocirrhosis and hepatitis diseases. (c) 2004 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | neural-network classification ; capillary-electrophoresis ; functional genomics ; modified nucleosides ; metabolome analysis ; gas-chromatography ; alignment ; profiles ; extracts ; urinary |
| 收录类别 | ISTP ; SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230862600031 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139722]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, J,Xu, GW,Zheng, WF,et al. Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry[J]. journal of chromatography a,2005,1084(1-2):214-221. |
| APA | Yang, J.,Xu, GW.,Zheng, WF.,Kong, HW.,Wang, C.,...&Pang, T.(2005).Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry.journal of chromatography a,1084(1-2),214-221. |
| MLA | Yang, J,et al."Strategy for metabonomics research based on high-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry".journal of chromatography a 1084.1-2(2005):214-221. |
入库方式： OAI收割
来源：大连化学物理研究所
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