On-column refolding and purification of recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) expressed as inclusion body in Escherichia coli
文献类型：期刊论文
| 作者 | Tan, HD; Dan, GP; Gong, HY; Cao, LJ |
| 刊名 | biotechnology letters
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 27期号:16页码:1177-1182 |
| 关键词 | human interleukin-1 receptor antagonist inclusion body on-column refolding |
| 英文摘要 | recombinant human interleukin-1 receptor antagonist (rhuil-1ra) was produced in e. coli as an inclusion body. rhuil-1ra was purified to over 98% purity by anion exchange chromatography after on-column refolding. the optimized processes produced more than 2 g pure refolded rhuil-1ra per 1 l culture, corresponding to a 44% recovery, without an intermediate dialysis step. refolded rhuil-1ra had full biological activity with the mtt assay. an intramolecular disulfide linkage in the oxidized recombinant protein was suggested by data from hplc and non-reducing sds-page. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | interleukin-1 receptor antagonist ; anakinra ; protein |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231856500005 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139785]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Yushui Biopharmaceut Co Ltd, Chongqing 200400, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, HD,Dan, GP,Gong, HY,et al. On-column refolding and purification of recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) expressed as inclusion body in Escherichia coli[J]. biotechnology letters,2005,27(16):1177-1182. |
| APA | Tan, HD,Dan, GP,Gong, HY,&Cao, LJ.(2005).On-column refolding and purification of recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) expressed as inclusion body in Escherichia coli.biotechnology letters,27(16),1177-1182. |
| MLA | Tan, HD,et al."On-column refolding and purification of recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) expressed as inclusion body in Escherichia coli".biotechnology letters 27.16(2005):1177-1182. |
入库方式： OAI收割
来源：大连化学物理研究所
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