Construction of a new method for mobilizing the zones of CIEF and its application
文献类型：期刊论文
| 作者 | Zhu, GJ; Yang, C; Liu, HC; Zhang, LH; Zhang, WB; Zhang, YK |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2005-04-10 |
| 卷号 | 26期号:4页码:654-656 |
| 关键词 | capillary isoelectric focusing(CIEF) electroosmotic flow(EOF) protein polypeptide |
| 英文摘要 | a simple method for mobilizing the focused zones of capillary isoelectric focusing (cief) for single-point detectors was developed, in which a linear polyacrylamide(lpa) coated capillary was connected with an uncoated capillary by an etched porous joint. the etched section allows electrical conductivity but blocks the hydrodynamic flow. the focused zones of mixed proteins was mobilized by this method successfully. compared with the traditional gravity mobilization step, it was found that this method can mobilize the focused zones more effectively without sacrificing separation efficiency. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | capillary-electrophoresis ; mass-spectrometry ; etched joint ; interface ; performance ; proteins |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228675700016 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139818]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, GJ,Yang, C,Liu, HC,et al. Construction of a new method for mobilizing the zones of CIEF and its application[J]. chemical journal of chinese universities-chinese,2005,26(4):654-656. |
| APA | Zhu, GJ,Yang, C,Liu, HC,Zhang, LH,Zhang, WB,&Zhang, YK.(2005).Construction of a new method for mobilizing the zones of CIEF and its application.chemical journal of chinese universities-chinese,26(4),654-656. |
| MLA | Zhu, GJ,et al."Construction of a new method for mobilizing the zones of CIEF and its application".chemical journal of chinese universities-chinese 26.4(2005):654-656. |
入库方式： OAI收割
来源：大连化学物理研究所
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