Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis
文献类型：期刊论文
| 作者 | Qin, JH; Liu, ZY; Wu, DP; Zhu, N; Zhou, XM; Fung, YS; Lin, BC |
| 刊名 | electrophoresis
 |
| 出版日期 | 2005 |
| 卷号 | 26期号:1页码:219-224 |
| 关键词 | angiotensinogen gene genotyping microchip electrophoresis miniaturization polymorphism analysis |
| 英文摘要 | angiotensinogen (agt) gene has been regarded as one of the candidate genes for essential hypertension. in our study, the role of agt gene as a putatively predisposing gene for hypertension was evaluated by genotyping a a (-6) g polymorphism in the core promoter region in 123 patients with essential hypertension and 103 healthy controls. a microchip electrophoresis method coupled with polymorphism chain reaction (pcr)-restriction fragment length polymorphism (rflp) assay was used for genotyping the a (-6) g single-nucleotide polymorphism. the separation and detection of the digested pcr amplicons were completed just in 280 s or less. the genotype frequency fulfilled the criteria of the hardy-weinbery equilibrium (x-2 = 3.067, p > 0.05). the results showed a higher frequency of the -6 a allele (0.70) in the normotensive subjects, which is higher than those reported in germany (0.47) and czech (0.40) populations, but similar to that found in japanese populations (0.73). the frequencies of genotype aa, ag, and gg were 0.46, 0.49, and 0.05 in hypertensive subjects, and 0.44, 0.53, and 0.03 in control subjects. there is no significant difference in the distributions of the genotype and allele between the two groups (x-2 = 0.88, p > 0.05; x-2 = 0.024, p > 0.05). these findings differ from some of the results obtained in other ethnic groups, indicating the potential importance of ethnic origin in the assessment of genetic risk identifiers for a complex disease. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | essential-hypertension ; m235t ; association ; variant ; dna ; linkage ; disease ; site |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000226467400028 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139844]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 1, Dept Pediat, Dalian, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 2, Dept Cardiol, Dalian, Peoples R China 4.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, JH,Liu, ZY,Wu, DP,et al. Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis[J]. electrophoresis,2005,26(1):219-224. |
| APA | Qin, JH.,Liu, ZY.,Wu, DP.,Zhu, N.,Zhou, XM.,...&Lin, BC.(2005).Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis.electrophoresis,26(1),219-224. |
| MLA | Qin, JH,et al."Genotyping the-6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis".electrophoresis 26.1(2005):219-224. |
入库方式： OAI收割
来源：大连化学物理研究所
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