K-ras gene mutation detection by single-strand conformation polymorphism with capillary electrophoresis
文献类型：期刊论文
| 作者 | Shi, XZ; Li, JH; Zhao, CX; Lu, S; Yang, Q; Xu, GW |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 33期号:2页码:177-180 |
| 关键词 | short-chain linear polyacrylamide capillary electrophoresis single-strand conformation polymorphism K-ras gene |
| 英文摘要 | mutation of k-ras gene plays an important role in colorectal cancer tumorigenesis. short-chain linear polyacrylamide (slpa) was synthesized using isopropanol as a chain transfer agent. the viscosity coefficient and molecular mass of slpa were 0.70 x 10(-3) m(3)/kg and 6.5 x 10(4) respectively. a single-strand conformation polymorphism with laser-induced fluorescence capillary electrophoresis method for detection of k-ras gene mutation was developed under the conditions of 6% slpa, running temperature at 27 degrees c running voltage at 9 kv. by this method, tumor tissues from 36 colorectal cancer patient were screened, and twelve of them were found to be k-ras gene mutation. the results revealed that this method is rapid and high sensitivity and become a promising tool to screen a large number of samples in clinical diagnosis of colorectal cancer. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | laser-induced fluorescence |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227651300008 |
| 公开日期 | 2015-11-10 |
| 源URL | [http://159.226.238.44/handle/321008/139847]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, XZ,Li, JH,Zhao, CX,et al. K-ras gene mutation detection by single-strand conformation polymorphism with capillary electrophoresis[J]. chinese journal of analytical chemistry,2005,33(2):177-180. |
| APA | Shi, XZ,Li, JH,Zhao, CX,Lu, S,Yang, Q,&Xu, GW.(2005).K-ras gene mutation detection by single-strand conformation polymorphism with capillary electrophoresis.chinese journal of analytical chemistry,33(2),177-180. |
| MLA | Shi, XZ,et al."K-ras gene mutation detection by single-strand conformation polymorphism with capillary electrophoresis".chinese journal of analytical chemistry 33.2(2005):177-180. |
入库方式： OAI收割
来源：大连化学物理研究所
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